Indonesian Journal of Forestry Research Vol. 8 No. October 2021, 159-171 ISSN: 2355-7079/E-ISSN: 2406-8195 FIXATION PROCESS OF LAMINATED BAMBOO COMPRESSION FROM CURVED CROSS-SECTION SLATS Teguh Darmawan*1,2. Adik Bahanawan2. Danang S. Adi2. Wahyu Dwianto2, and Naresworo Nugroho*1 Faculty of Forestry. IPB University. Jl. Raya Dramaga. IPB Campus Dramaga Bogor, 16680 West Java. Indonesia Research Center for Biomaterials. Indonesian Institute of Sciences Jl. Raya Bogor Km. Bogor 16911. West Java. Indonesia Received: 27 November 2020. Revised: 27 June 2021. Accepted: 4 July 2021 FIXATION PROCESS OF LAMINATED BAMBOO COMPRESSION FROM CURVED CROsECTION SLATS. Removing the outer part of bamboo for manufacturing flat bamboo lamination has disadvantage on the density of the product. The purpose of this experiment was to investigate the fixation of compressed bamboo from curved cross-section slats. The compression of bamboo slats using densification technique was aimed for uniform density. Furthermore, steam treatments were conducted to fix the deformation. The compressed bamboo slats revealed that the density of the samples at the bottom parts increased from 0. 4Ae0. 56 g/cm3 to 0. 89Ae1. 05 g/cm3 after pressing with a compression level between 98Ae63. 97%, while the samples in the middle parts increased from 0. 7Ae0. 83 g/cm3 to 1. 02Ae1. 18 g/cm3 with the compression level of 32. 92Ae41. These results were slightly higher than that of the upper parts, which was between 0. 91Ae0. 98 g/cm3. The recovery of set decreased and the weight loss increased with increasing temperature and steam treatment time. Fixation of compressive deformation could be achieved at 160AC within 60 minutes. The bottom parts of samples experienced a slightly greater weight loss compared to the middle parts, i. 38% and 7. 49%, respectively. The anatomical structure of bamboo tended to deform during densification process. Furthermore, the steam treatments affected the colour of densified bamboo which became darker. From this experiment, it can be concluded that the manufacture of laminated bamboo from bamboo slats can be uniformed in strength by equalizing the density at the bottom and middle with the upper parts through the densification technique. However, further research should be conducted to know the delamination and shear strength of the bamboo lamination. Keywords: Laminated bamboo, curved cross-section slats, fixation process, steam treatment PROSES FIKSASI KOMPRESI BAMBU LAMINASI DARI BILAH BERPENAMPANG LENGKUNG. Penghilangan bagian luar dari bambu pada pembuatan laminasi berbentuk datar mempunyai kelemahan pada kerapatan Tujuan penelitian ini adalah untuk meneliti fiksasi bambu kompresi dari bilah berpenampang lengkung. Pengepresan bilah bambu dengan teknik densifikasi ditujukan untuk menyeragamkan kerapatannya. Selanjutnya, perlakuan uap panas dilakukan untuk mempertahankan deformasinya. Bilah bambu yang dikompresi tersebut menunjukkan bahwa kerapatan contoh uji di bagian bawah meningkat dari 0,4Ae0,56 g/cm3 menjadi 0,89Ae1,05 g/cm3 setelah dipres dengan tingkat kompresi antara 46,98Ae63,97%, sedangkan contoh uji di bagian tengah meningkat dari 0,7Ae0,83 g/cm3 hingga 1,02Ae1,18 g/cm3 dengan tingkat kompresi antara 32,92Ae41,5%. Hasil ini sedikit lebih tinggi dari pada di bagian ujung, yaitu antara 0,91Ae0,98 g/cm3. Pemulihan ketebalan menurun dan kehilangan berat meningkat dengan meningkatnya suhu dan waktu perlakuan uap panas. Fiksasi dari deformasi tersebut dapat dicapai pada 160AC dalam waktu 60 menit. Contoh uji bagian bawah mengalami kehilangan yang sedikit lebih besar dibandingkan dengan bagian tengah, yaitu masing-masing 8,38% dan 7,49%. Struktur anatomi bambu cenderung berubah bentuk selama proses densifikasi. Selanjutnya, perlakuan uap panas berpengaruh pada warna bambu kompresi yang menjadi lebih gelap. Dari penelitian ini dapat disimpulkan bahwa pembuatan bambu laminasi dari bilah bambu dapat diseragamkan kekuatannya dengan menyamakan kerapatan di bagian bawah dan tengah dengan bagian ujungnya melalui teknik densifikasi. Namun, penelitian selanjutnya perlu dilakukan untuk mengetahui delaminasi dan keteguhan geser dari bambu laminasi tersebut. Kata kunci: Bambu laminasi, bilah berpenampang lengkung, proses fiksasi, perlakuan uap panas Corresponding author: naresworo@yahoo. darmawan@biomaterial. A2021 IJFR All rights reserved. Open access under CC BY-NC-SA license. doi:10. 20886/ijfr. Indonesian Journal of Forestry Research Vol. No. October 2021, 159-171 INTRODUCTION The high rate of population growth in Indonesia undoubtedly leads to increasing housing demand. With the expected high demand for housing, it is necessary to provide adequate raw material. Bamboo can be an alternative raw material to diversify non-timber building materials to substitute wood-based building materials which availability decreases. Bamboo material is abundant and can be obtained easily in almost areas in Indonesia with a total production of more than 20. million culms in 2018 (Statistics Indonesia. It has a short harvesting age of 3Ae5 years (Hastuti. Primairyani, & Ansori, 2018. Rahmawati. Baharuddin, & Putranto, 2. , and prices are relatively cheaper compared to wood. The strength value of bamboo is equivalent to strength class i of wood, even the strength of the board from processed bamboo products can reach strength class IAeII (Li. Zhang. Huang, & Deeks, 2013. Mahdavi. Clouston, & Arwade, 2012. Nugroho & Ando, 2000, 2001. Sulastiningsih & Nurwati. Based on these considerations, bamboo products' development has strategic value, and it is important to continue to be pursued. However, bamboo morphology is not as solid as wood because it has a cavity in the centre of the bamboo stem. Therefore, the bamboo utilisation needs further treatment, especially for the structural application, for instance, using the lamination technique. ISSN: 2355-7079/E-ISSN: 2406-8195 In the laminated bamboo manufacture, bamboo slats are generally made in a flat square shape on each side. However, it has several disadvantages because the bamboo slats' crosectional size depends on the bamboo stem's Cutting into a square shape will be effective only for bamboo species with thick walls (Sulastiningsih & Nurwati, 2009. Sutiyono. Generally flat cross-section lamination is made by removing the outer part of the bamboo (Figure 1. , which has a higher density than the inner part. However, this method is low in recovery and it is considered as unefficient. The weaknesses in the manufacture of the laminated bamboo can be reduced by utilizing the bamboo slats that still maintain their curved Curved slats will increase the yield of laminated bamboo, usually 7Ae13% higher than those with square cross-sections (Budiana. Kusmawan, & Rusli, 2. Besides, the curved cross-section has a better mechanical advantage than the square one due to the cross-section's larger modulus, more resistant to loading and more rigid as reported by Mujiman et al. The manufacture of laminated bamboo from curved cross-section bamboo slats has been carried out in preliminary research (Figure However, the bamboo lamination was not considered among the factors which affected the manufacturing, such as density. This is a major problem because it will result in non-uniform density and strength of laminated bamboo. Figure 1. Bamboo slats with square . and curved . cross-sections Fixation Process of Laminated Bamboo Compression from Curved Cross-Section Slats. (Teguh Darmawan et al. On the other hand, the density of bamboo increases from the bottom to the upper parts of the stem and from the inner to the outer parts (Rosalita. Nugroho. Subiyanto, & Kusumah. Yu. Jiang. Hse, & Shupe, 2. Dwianto et al. reported the density of 3 years old andong bamboo (Gigantochloa pseudoarundinacea (Steud. ) Widjaj. , which was taken from the 1st to 20th internodes, increased from 0. 48 g/cm3 at the bottom to 0. 74 g/cm3 at the upper parts. These are influenced by the portion between the vascular bundles and its parenchyma cells. The strength characteristics also have the same pattern because the strength of a material is greatly influenced by its density (Dwianto et al. Therefore, an effort should be conducted to uniform the density by using densification The bottom parts of the bamboo slats with lower densities can be compressed to match the upper parts' density. The densification process in the radial direction of the bamboo slats is possible because the inner parts of the bamboo consist of parenchyma cells that have thin cell walls. This phenomena is similar with the wood densification process, where the deformation starts from the thin cell walls in the early wood (Laine. Segerholm. Rautkari. Wa, & Hughes, 2. In general, the bigger diameter of the bamboo is preferable for this utilization. The diameter of the bamboo decreases from the bottom to the upper parts. It is believed that the bigger the diameter, the higher the proportion of parenchyma cell. Therefore, densification of the big diameter of bamboos is required to optimize the application. However, the recovery to the initial shape becomes one of the issues of the compression techniques. In order to prevent recovery of the deformation from the densification process due to the influence of moisture, a permanent fixation treatment is required. There have been many permanent fixation treatments for wood densification (Amin & Dwianto, 2006. Bao. Huang. Jiang. Yu, & Yu, 2017. Darwis. Wahyudi. Dwianto, & Cahyono, 2017. W Dwianto. Morooka. Norimoto, & Kitajima, 1999. Inoue. Norimoto. Tanahashi, & Rowell, 1993. Kariz et al. , 2017. Zhan & Avramidis, 2. , while bamboo was less of a concern (Subiyanto. Dwianto, & Higashihara, 2. Inoue et al. stated that permanent fixation of wood densification could be achieved by saturated steaming at a temperature of 180AC for 8 minutes or 200AC for 1 minute. These results were not much different from the research of Navi and Heger . who reported that fixation could be achieved by saturated steam treatment at 165AC for 30 minutes, 190AC for 8 minutes, or 200AC for 2 minutes. Dwianto et al. investigated that the permanent fixation was caused by stress relaxation of the internal The development of a method through post-treatment of compressed wood was able to produce permanent fixation (Antikainen et al. , 2014. Laine. Rautkari, & Hughes. Zhan & Avramidis, 2. Wood and bamboo are lignocellulosic materials. bamboo fixation treatment may not be much different from wood. Therefore, this study was conducted to investigate the fixation process of compressed laminated bamboo from curved cross-section slats. II. MATERIAL AND METHODS Materials and Morphological Characterization of Bamboo Stem This experiment used 3 stems of 3 yearsold sembilang (Dendrocalamus giganteus Munr. bamboo which is usually utilized as a structural material in Indonesia. The samples were taken from a bamboo collection garden at the Research Center for Biomaterials LIPI. Cibinong. The morphological characterizations of bamboo stems included the measurements outer diameter and stem thickness. Densification Technique and Fixation Treatment of Bamboo Slats The bottom parts of the bamboo slats were taken from internode number 1 to 3 with an average thickness of 15 mm, the middle parts were taken from internode number 10 to 13 Indonesian Journal of Forestry Research Vol. No. October 2021, 159-171 with an average thickness of 7. 5 mm, and the upper parts were taken from internode number 31 to 40 with an average thickness of 4. 5 mm. The target of each compression of bamboo was uniform density. To get the same target density, the bottom parts of the bamboo slats were arranged in 4 layers, the middle parts were arranged in 6 layers, while the upper parts were arranged in 6 layers, also. Therefore, the compression target of 50% was conducted for the bottom and 30% for the middle parts, and was expecting attaining the same density with the upper parts. The bamboo slats were prepared in wet condition. they were arranged in a moulding equipped with a lock/clamp and pressed until reached an approximate thickness of 3 cm (Figure . It was also reported that the higher the temperature, the lower the time required to reach the fixation. However, it has a negative effect on the weight loss, which has linear relationship with the time. Therefore, this experiment was conducted with lower temperature, to reduce the weight loss during the fixation process. The compressed bamboo slats were then put into a chamber in clamped condition and subjected to steam treatment at temperatures of 120AC, 140AC and 160AC for 30 and 60 minutes. After that, they were oven-dried at 100AC for 24 hours. At this stage, the measurement of changes in the test samples' weight before and after the steam treatment was carried out. ISSN: 2355-7079/E-ISSN: 2406-8195 Analysis of Physical Properties The testing of physical properties of bamboo slat samples included density, recovery of set, and weight loss. The density measurement referred to the ISO 22157-1 . The test samples were initially weighed (W. and their volumes were measured (V. , then ovendried at 100-105AC for 24 hours or until their weight was constant (W. The determination of density was calculated by equation 1. Density = . The thickness recovery measurement method referred to Amin and Dwianto . to determine the fixation of compressed bamboo Before compressed, all the bamboo slats were dried in the oven for 3 days at 60AC, and the thickness (T. and initial weight (W. was The bamboo slats were arranged on the moulding, then compressed and subjected to steam treatment. Furthermore, all the samples were oven-dried and the thickness (T. was measured. The recovery test was carried out with soaking in room temperature water for 24 hours and boiling for 30 minutes. The boiled bamboo slats were dried again in the oven, then the thickness (T. and the final weight slats (W. were measured. The recovery of set (RS) and weight loss (WL) were measured by Formula 2 (Tr Oe T. RS % = x100 . (To Oe T. (Wo Oe Wr ) WL% = x100 . Observation of Anatomical Structures Figure 2. Laminated bamboo moulding for steam The anatomical observation was conducted on three different parts, i. bottom, middle and upper. The samples were 1 cm . x 1 cm . , while the thicknesses were based Fixation Process of Laminated Bamboo Compression from Curved Cross-Section Slats. (Teguh Darmawan et al. on their original stem thickness. The analysis was on the cross section of the samples before and after compression and steam treatment by stereo microscope Keyence VHX 6000. RESULT AND DISCUSSION Morphological Characterization of Bamboo Stem Outer diamter . Stem thickness . The stem of bamboo is straight and cylinderformed with nodes, and the parts between nodes are called internodes (Zhang. Jiang, & Tang. Every node has two closely positioned The part between the rings is the node There is a wooden partition between two neighboring internodes, which strengthens the The length of internodes, the number and form of nodes depend on the species of The internodes are hollow inside, which forms bamboo cavities. The thickness of stem wall varies greatly following different bamboo species. Morphological characteristics of the outer diameter, stem thickness and density of each internode of sembilang bamboo were analysed to determine the causes of differences in their density distribution pattern. Figure 3 shows the average outer diameter . , stem thickness . , density . , and the relationship between stem thickness and density . of sembilang The outer diameter of sembilang bamboo decreased from an average of 16 cm at the bottom to about 7 cm at the upper parts of the stem following the polynomial equation of y = -0. 0039x2 - 0. RA = 0. (Figure 3. It shows that laminated bamboo Internode number . Density . /cm. Density . /cm. Internode number Internode number Stem thickness . Figure 3. Outer diameter . , stem thickness . , density . , and the relationship between stem thickness and density . of sembilang bamboo Indonesian Journal of Forestry Research Vol. No. October 2021, 159-171 with a curved cross-section width of 5 cm can be made using sembilang bamboo up to the 40th The stem thickness of sembilang bamboo decreased drastically from an average of 1. cm at the bottom to the 20th internode to about 6 cm at the upper parts of the stem following the logarithmic equation of y = -0. RA = 0. 9902 (Figure 3. The difference in the stem thickness in each internode from bottom to the upper parts greatly affected the density. It can be seen that the density of sembilang bamboo increased from an average 4 g/cm3 at the bottom to almost 1. 0 g/cm3 at the upper parts of the stem following the polynomial equation of y = 5e-05x2 0. ISSN: 2355-7079/E-ISSN: 2406-8195 RA = 0. 943 (Figure 3. The average density decreased with stem thickness following the equation of y = 0. 6697xAe0. RA = 0. (Figure 3. The density of bamboo depended on their anatomical structures. Zhang et al. reported that bamboo's basic density was in the range of 0. 4Ae0. 8 g/cm3. They argued that bamboo's basic density mainly depended on the density of vascular bundles and their As a rule, the bamboo stem density increased from inner to the outer parts and from the lower to the upper parts. Kabir. Bhattacharjee, and Sattar . Patel et al. Sattar. Kabir, and Bhattacharjee . reported that the density of bamboo Table 1. Density increment, weight loss and recovery of set of compressed sembilang bamboo slats by steam treatment Treatment Temp Time (AC) Density Target Actual Before After Weight loss Recovery of Set . (%) (%) . /cm. /cm. (%) (%) Bottom 46 . Middle 79 . Upper 60 . Bottom 44 . Middle 22 . Upper 87 . Bottom 66 . Middle 05 . Upper 18 . Bottom 81 . Middle 62 . Upper 17 . Bottom 19 . Middle 96 . Upper 26 . Bottom 59 . Middle 26 . Upper 29 . Position . Compression Stem Note: The value of the numbers in parentheses is the standard deviation Fixation Process of Laminated Bamboo Compression from Curved Cross-Section Slats. (Teguh Darmawan et al. varied from 0. 50 to 0. 80 g/cm3 depending on anatomical structures such as quantity and distribution of fibres around vascular bundles, with its maximum density usually obtained from 3 years old mature culms. at temperatures of 120A, 140A, and 160AC for 30 and 60 minutes to determine the recovery of set or level of fixation. Furthermore, the increase in density and weight loss due to the steam treatment was measured. Figure 4 shows relationship between density and compression level . and steam treatment . Compression target was 50% for the bottom and 30% for the middle parts. the compression levels were mostly higher. This was probably due to shrinkage of the samples in radial direction. The results showed that the density of the bottom parts increased from 40Ae0. 56 g/cm3 to 0. 89Ae1. 05 g/cm3 at the compression level of 46. 98Ae63. On the other hand, the middle parts increased from 70Ae0. 83 g/cm3 to 1. 02Ae1. 18 g/cm3 at the compression level of 32. 92Ae41. 50% (Figure The increase in density at the bottom and middle parts was slightly higher than that of the upper parts, which was between 0. 91Ae0. g/cm3 (Figure 4. Meanwhile, the increase in steaming temperature and time did not significantly affect the increase in density. The recovery of set decreased with increasing steam temperature and time (Figure The bottom part samples underwent a slightly higher thickness recovery than the Equalization of Density and Fixation of Compressed Bamboo Slats Remarks: Density . /cm. Density . /cm. Table 1 shows the average results of bamboo slats' physical properties before and after compression and steam treatment. The stem thickness of the sembilang bamboo was very different between bottom, middle, and upper parts, which were 13. 81Ae16. 19 mm, 6. 96Ae8. mm, and 4. 17Ae4. 87 mm, respectively. This trend was similar with the densities which were 0. 40Ae 56 g/cm3, 0. 7Ae0. 83 g/cm3, and 0. 83Ae0. 96 g/ cm3, respectively. Therefore, the compression target in this experiment corresponded to their thicknesses which were 50% for the bottom and 30% for the middle. The compression targets were intended to equalize the density between the bamboo slats at the bottom and middle with the upper parts. The upper parts did not determine the compression target but undergoes compression when equalizing the curved lines so that the density increased The steam treatment was conducted Compression level (%) Steaming temperature and time Remarks: Figure 4. The relationships between density and compression level . and steam treatment . Recovery of set (%) Temperature (AC) Temperature (AC) Remarks: ISSN: 2355-7079/E-ISSN: 2406-8195 Weight loss (%) Indonesian Journal of Forestry Research Vol. No. October 2021, 159-171 Remarks: Recovery of set (%) Figure 5. Relationship between recovery of set . and weight loss . with temperature and time of steam Remarks: Weight loss (%) Figure 6. Relationship between recovery of set and weight loss middle part samples. However, fixation could be achieved by both parts of samples at the same steam temperature and time, i. within 60 minutes. The weight loss increased with increasing steam temperature and time (Figure 5. , especially at 160AC. Although it was not too different, it appeared that the samples at the bottom lost more weight than the middle and upper parts. then the samples at the upper parts lost least weight. Figure 6 shows the relationship between recovery of set and weight loss. the lower the fixation, the greater the weight loss was. Therefore, it was assumed that the fixation was influenced by the magnitude of the weight loss. Besides, it can be seen that the bottom part samples experienced greater recovery of set and weight loss than the middle part samples. This was indicated by separate equation between the bottom, i. y = 17. 174x2 - 287. RA = 0. 7957 and the middle part samples, i. Fixation Process of Laminated Bamboo Compression from Curved Cross-Section Slats. (Teguh Darmawan et al. y = 3. 3206x2 - 71. RA = 0. However based on this experiment, the fixation level would be achieved when the weight loss 38% and 7. 49% at the bottom and middle parts, respectively. The anatomical structure of bamboo is different in each position. Generally, the bottom parts of bamboo have a greater proportion of vessel cell, higher lumen diameter, and thicker cell wall than those of middle and upper parts. As a lignocellulosic material, bamboo consists of cellulose (A55%), hemicellulose . entosan A20%), and lignin (A25%) in the complex structure (Chen, 2015. Dotan, 2014. Khalil et al. , 2012. Li. Wang. Wang. Cheng, & Han. However, the minor chemical compound on the bamboo species, such as pigment, tannins, protein, fat, was higher than those of wood (Fazita et al. , 2. Since the anatomical structural were different on the axial direction, the chemical components, such as holocellulose and extractive contents are also different in these third parts. Holocellulose is a total fraction of the carbohydrate, which consist of cellulose and hemicellulose. Besides, hemicellulose is a low molecular polymer sensitive to temperature (Murda et al. , 2. Moreover. Esteves and Pereira . reported that hemicellulose was the first degraded wood component when subjected to heat treatment. Loiwatu and Manuhuwa . reported that extractive contents on the bottom, middle, and upper parts were 6. 44%, and 5. 43%, respectively. Extractive contents were dominant in the cell lumen, and also in the cell wall. Extractive contents, such as tannin, resin, and volatile compound, were also degraded by heat or steam treatment. Therefore, densification process on the bottom parts at 160AC steam treatment for 60 minutes has higher weight loss than that of the middle Subiyanto et al. conducted research on the permanent fixation of radially compressed Tali and Gombong bamboos in dry condition by heat treatment. The results showed that the recovery of the set at 160AC was around 35Ae40% even when they were heated for more than 12 hours. In addition, the fixation was achieved at 180AC for 8 hours and 200AC for 4 hours. the weight loss was around 6Ae8% of both treatments. This weight loss was similar with our study by using steam treatment at 160AC for 60 minutes . as the optimal The densification of bamboo with steam treatments below 160AC has not occurred yet due to the existence of internal stress stored in the bamboo structure during the deformation. Fang. Mariotti. Cloutier. Koubaa, and Blanchet . stated that internal stress was stored in the semi-crystalline cellulose, hemicellulose, and This stress can be reduced or released by applying the heat or steam treatment (Dwianto et al. , 1999. Dwianto. Morooka, & Norimoto. They reported that the permanent fixation of wood compression by heat or steam treatments was caused by the release of stress stored in microfibrils and hemi-lignin matrix due to the degradation of polymers in wood cell walls as the decomposition of hemicellulose and lignin. The relaxation of internal stress by steam treatment caused the changes in the composition of wood and created cross-links on lignin to reduce wood hygroscopicity (Guo. Yin. Zhang. Salmyn, & Yin, 2017. Obataya & Chen, 2. Observation of Anatomical Structures The tissues of bamboo stem include surface system . pidermis, subcutis, corte. , entire system . undamental tissues, pith rings, pith. and vascular system (Zhang et al. , 2. The surface system is bamboo skin, located in the outermost part, pith rings and piths located in innermost part. They form the stem wall's outer and inner surface layers, protecting closely the fundamental tissues and vascular Vascular bundles are distributed among the stem wall's fundamental tissues, and their density decreases from the outer part of the stem wall to the inner part. Fundamental tissue is parenchyma cells, mainly distributed within the vascular system as a stuffing material. These Indonesian Journal of Forestry Research Vol. No. October 2021, 159-171 ISSN: 2355-7079/E-ISSN: 2406-8195 Table 2. Number and frequency of vascular bundles and percentage of fiber bundles in sembilang bamboo Observation Bottom Sampling area . m ) 409 . Number of vascular bundles . Frequency of vascular bundles . nit/mm ) 2 . Percentage of fiber bundles (%) 78 . Sample position Middle Upper 876 . Note: The value of the numbers in parentheses is the standard deviation . Figure 7. Anatomical structures of cross-section uncompressed and compressed bamboo at the bottom . , middle . , and upper parts . are the fundamental part of bamboo material. Most of the cells are larger, with a thinner wall. The thickness of bamboo skin . utermost par. and pith . nnermost par. as surface layers seemed unchangeable from bottom to the upper part of bamboo culms. Therefore, the stem wall thickness changes were mostly due to the portion changes of parenchyma cells and vascular bundles, which were distributed along the stem wall. The density of vascular bundles decreased from the outer part of the stem wall to the cross-section's inner part. Table 2 shows the number and frequency of vascular bundles and percentage of fibre bundles in the bottom, middle and upper parts of sembilang bamboo. The frequency of vascular bundles and percentage of fibre bundles increased by increasing height of the These unique characteristics of the cell compositions of the bamboo were assumed to affect the difference of density in the axial Figure 7 shows anatomical structures of cross-section uncompressed and compressed bamboo at the bottom . , middle . , and upper parts . The anatomical structures of bamboo tended to deform during the densification This process caused deformation on the parenchyma and vessel cells in the bottom and middle parts of bamboo, then, the vascular bundles appeared denser. The deformation occurred on the weakest structures, such as parenchyma cells, because having thinner cell walls than the other cells (Darwis et al. Laine et al. , 2. Vascular bundles of densified bamboo on the bottom-inner parts Fixation Process of Laminated Bamboo Compression from Curved Cross-Section Slats. (Teguh Darmawan et al. were flat. These shapes were not found in the other parts, especially in middle parts. In this region, the shapes of vascular bundles were remained unchanged from the original one, but those vessels were closer. Besides, the steam treatment affected the colour of the densified bamboo and became darker. IV. CONCLUSION From the measurement of morphological characteristics, it can be concluded that sembilang bamboo has the advantage of a larger The density of bamboo slats at the bottom parts increased from 0. 40Ae0. 56 g/cm3 89Ae1. 05 g/cm3 at the compression level of 98Ae63. Furthermore, the middle parts increased from 0. 70Ae0. 83 g/cm3 to 1. 02Ae1. 18 g/ cm3 at the compression level of 32. 92Ae41. The density of the middle parts was slightly higher than that of the upper parts, which was 91Ae0. 98 g/cm3. The recovery of set decreased and the weight loss increased with the increasing temperature and steam treatment Fixation could be achieved at 160AC within 60 minutes. To achieve the fixation, the bottom parts of the samples experienced a slightly greater weight loss than the middle parts, i. 38% and 7. 49%, respectively. The anatomical structure of bamboo slats tended to deform during the densification This process caused by deformation on the parenchyma and vessel cells in the bottom and middle parts, then the vascular bundles appeared denser and closer than the initial Furthermore, the steam treatment affected the colour of densified or compressed bamboo, which became darker. From this experiment, it can be concluded that the manufacture of laminated bamboo from bamboo slats can be uniformed in strength by equalizing the density at the bottom and middle with the upper parts through the densification However, further research should be conducted to know the delamination and shear strength of the bamboo lamination. ACKNOWLEDGEMENT This research is regarded as partial fulfilment of the Magister Program by Research from the Indonesian Institute of Sciences-IPB University. This work was supported by the DIPA and PRN-LPDP Program 2020. The authors would like to thank the Head of the Research Center for Biomaterials. Indonesian Institute of Sciences for providing support and facilities in this research and to fellow REFERENCES