Open Global Scientific Journal 5 . : 41-53 2026 Contents lists available at openscie. E-ISSN: 2961-7952 Open Global Scientific Journal DOI: 10. 70110/ogsj. Journal homepage: https://openglobalsci. Lead (P. Accumulation by Typha angustifolia and Cyperus alternifolius in a Constructed Wetland System Nilam Kinanti1. Intan Permata Hadi2* Department of Soil Science. Faculty of Agriculture. Brawijaya University. Malang. East Java. Indonesia Department of Soil Science. Faculty of Agriculture. Mataram Univesity. West Nusa Tenggara. Indonesia *Correspondence E-mail: intanpermatahadi1@staff. ARTICLE INFO ABSTRACT Background: Lead (P. is one of the most common heavy metal contaminants in aquatic environments due to its extensive use in industrial In developing countries such as Indonesia, wastewater treatment facilities often have limited capacity to effectively process industrial effluents, leading to the release of Pb-contaminated wastewater into the environment. Aims: This study aimed to evaluate the Pb accumulation potential and Keywords: phytoremediation performance of Typha angustifolia and Cyperus Bioconcentration factor, alternifolius in a laboratory-scale constructed wetland system operated Constructed wetland, under a static batch method for the remediation of Pb-contaminated water. Cyperus alternifolius. Methods: The experiment consisted of a control treatment without plants Translocation factor, and two plant species exposed to three Pb concentrations . , 20, and 30 Typha angustifolia. mg LAA). Water temperature, water pH, soil pH, and Pb concentrations in water, soil, and plant tissues were measured. Temperature and pH were recorded at 1 and 14 days after planting, while Pb concentrations were analyzed at the end of the 14-day experimental period. The phytoremediation potential of both plant species was evaluated using the Bioconcentration Factor (BCF) and Translocation Factor (TF). Results: The results showed that the constructed wetland system effectively reduced Pb concentrations in water, with removal efficiencies ranging from 85% to 96%. However, the soil have big contribution in removal Pb in water. angustifolia demonstrated BCF and TF values greater than 1 across all Pb concentrations, indicating strong accumulation and translocation abilities. In contrast. alternifolius exhibited BCF values below 1 but TF values greater than 1, suggesting limited accumulation capacity with effective Pb translocation from roots to Conclusion: Both species have potential for application in constructed wetland systems for Pb remediation, with T. angustifolia showing superior phytoremediation performance. To cite this article: Kinanti. Hadi. Lead (P. accumulation by Typha angustifolia and Cyperus alternifolius in a constructed wetland system. Open Global Scientific Journal, 4. , 41Ae53. Article History: Received 27 April 2026 Revised 3 June 2026 Accepted 5 June 2026 Published 7 June 2026 This article is under a Creative Commons Attribution-ShareAlike 4. 0 International (CC BY-SA 4. License. Creative Commons Attribution-ShareAlike 4. 0 International License Copyright A2026 by author/s Introduction Heavy metal pollution is one of the environmental problems that receives a lot of attention due to its persistent nature, difficulty to degrade, and ability to accumulate in various environmental compartments such as water, soil, and sediments. One of the most commonly found heavy metals in the environment is lead (P. Lead is a non-essential metal that has no biological function for organisms but can be toxic when accumulated at certain concentrations. The presence of Pb in the environment generally originates from anthropogenic activities such as industrial activities, the use of leaded fuel, agricultural activities, and the disposal of poorly managed waste (Sharifi et al. , 2. The widespread use of Pb in various sectors has led to increased distribution and accumulation of this metal in the environment over time (Erfandi & Juarsah, 2. The accumulation of Pb in the environment has become a serious concern because this metal can enter the food chain through contaminated soil and water media. In addition, the limited capacity for waste treatment in some industrial sectors has resulted in the continued occurrence of waste disposal that does not meet environmental quality standards (Kumar & Choudhary, 2. Intensive agricultural activities are also reported to contribute to the increase in heavy metal accumulation through the use of fertilizers and pesticides, accounting for about 69. 7Ae86. 7% of heavy metal input into the environment (Wang et , 2. Rahayu et al. reported that soil in agricultural land in Poncokusumo District. Malang Regency contained Pb at 7. 86Ae10. 20 mg. kgAA, while cultivated vegetable plants showed Pb content of 51Ae31. 72 mg. kgAA, exceeding the maximum limit according to the Indonesian National Standard, which is 0. 5 mg. kgAA. This condition indicates the need for effective technology to reduce Pb These conditions indicate the need for effective technology to reduce Pb concentration in the environment. One of the technologies developing for waste treatment and heavy metal remediation is Constructed Wetland (CW) or artificial wetlands. Constructed Wetland is an engineered system that mimics natural mechanisms in wetland ecosystems by utilizing the interaction between plants, growing media, and microorganisms in removing pollutants (Ioannidou et al. , 2. This technology is widely used because it is relatively inexpensive, environmentally friendly, easy to operate, and capable of reducing various types of pollutants including heavy metals. In this system, plants play an important role in the phytoremediation process through mechanisms of absorption, accumulation, and translocation of contaminants in plant tissues. The selection of plant species is an important factor in determining the effectiveness of the phytoremediation process. Plants with rapid growth, high biomass, and tolerance to polluted environments generally show better remediation potential (Malik et al. , 2. Typha angustifolia and Cyperus alternifolius are wetland plants that are tolerant of waterlogged conditions and have been reported to have the ability to absorb heavy metals. Previous studies have shown that T. angustifolia can accumulate Pb at 43. 6 mg. kgAA (Birgani et al. , 2. , while C. alternifolius has a Pb accumulation capacity of around 9 mg. kgAA (Nokande et al. , 2. Various studies on the utilization of wetland plants for heavy metal remediation have been conducted, but research specifically comparing the Pb accumulation capacity of T. angustifolia and C. in Constructed Wetland systems is still relatively limited. Most previous studies focused more on the effectiveness of pollutant removal in general or the use of a single plant species. Therefore, this study was conducted to evaluate the accumulation capacity of T. angustifolia and C. alternifolius in absorbing Pb using a Constructed Wetland system and to analyze the phytoremediation potential of both species based on Bioconcentration Factor (BCF) and Translocation Factor (TF) values. The novelty of this study lies in the comparative assessment of Pb accumulation and translocation by Typha angustifolia and Cyperus alternifolius within a static batch constructed wetland system. The results of this study are expected to provide information on the potential of both plants as alternative phytoremediators in the treatment of water contaminated with heavy metals. Methods The study was conducted using a laboratory-scale constructed wetland system operated under a static batch method for the treatment of Pb-contaminated water. In this system, contaminated water was introduced into the treatment reactors and maintained throughout the experimental period without continuous inflow and outflow. The reactor consisted of cylindrical plastic containers with a diameter of 30 cm and a height of 50 cm. Soil media were added into each reactor up to a height of 15 cm according to USEPA . , resulting in a soil volume of approximately 10. 6 L per reactor. The experimental period was conducted for 14 days, as previous studies have shown that Pb accumulation and physiological responses to heavy metal exposure in aquatic organisms (Li et al. , 2. and macrophytes can be effectively observed within this duration (Bordon et al. , 2. The soil used in this study was collected from Wajak District. Malang Regency. East Java. Indonesia. Initial soil characterization included soil texture analysis using the hydrometer method, soil pH measurement using a pH meter (HCCO), organic carbon analysis using the Walkley and Black method, and cation exchange capacity (CEC) analysis using NHCEOAc extraction at pH 7. The reactor design used in this study is presented in Figure 1. Figure 1. Reactor Tank Design Water temperature, water pH, soil pH, and Pb concentrations in water, soil, and plant tissues were measured during the experiment. Temperature and pH were recorded at 1 and 14 days after planting, while Pb concentrations in water, soil, and plant samples were analyzed at the end of the 14-day experimental period. Pb concentrations were expressed as mg LAA for water samples and mg kgAA dry weight for soil and plant samples. Plant samples were harvested at the end of the experiment and separated into roots and shoots. The samples were washed with distilled water to remove adhering particles, air-dried for 2Ae3 days, and ground to obtain a homogeneous material. The powdered samples were further pulverized using a mortar and pestle and passed through a 600-mesh sieve before being oven-dried at 105 AC. Approximately 5 g of each sample was transferred into a beaker glass, mixed with 50 mL distilled water, 5 mL concentrated HNOCE, and 2 mL concentrated HCl, and then digested on a hot plate for 2Ae3 h until a clear solution was After cooling, the digest was filtered through filter paper and transferred into a 50 mL volumetric flask. Soil samples were air-dried and sieved prior to analysis. The concentrations of Pb in water, soil, and plant samples were subsequently determined using an Atomic Absorption Spectrophotometer (AAS. Shimadzu AA-7000. Japa. angustifolia and C. alternifolius plants were washed with tap water to remove soil particles attached to the roots before transplantation. Plant selection was carried out to obtain uniform plant conditions. angustifolia plants with a height greater than 50 cm and without flowering structures were selected. Meanwhile. alternifolius plants were selected based on non-flowering conditions or flowers that had not yet changed to brown color, indicating that the plants had not reached full maturity. Each reactor was planted with 4Ae7 individuals with a similar fresh weight of approximately A100 g per treatment (Wang et al. , 2. Initial plant characteristics including plant height, root length, and leaf number were recorded prior to treatment. Synthetic wastewater was made using Merck brand lead nitrate (Pb(NOCE)CC) powder (Merck 107398. German. A stock solution . 0 mg/L) was prepared by dissolving 1. 598 grams of Pb(NOCE)CC in 1 liter of distilled water. Then, the stock solution was taken and diluted according to the concentration to be used (Githuku et al. , 2. This study used a Randomized Block Design (RBD) consisting of nine treatments with three replications. The following are the treatments and research parameters (Figure 2 and Table . Figure 2. Research Treatment and Layout Table 1. Research parameters Parameters Water temperature Water pH Soil pH Pb in Water Pb in Soil Pb in plants Note: DAP: Days After Planting Unit mg LAA mg LAA mg LAA Observation Time 1 DAP dan 14 DAP 1 DAP dan 14 DAP 1 DAP dan 14 DAP 14 DAP 14 DAP 14 DAP Next, the data were analyzed using one-way analysis of variance (ANOVA) with an F test at a 5% level and further test DMRT (DuncanAos Multiple Range Tes. using IBM SPSS Statistics 24 and Microsoft Excel software. The potential of plants in accumulating heavy metals in tissues was determined by calculating the Bioconcentration Factor (BCF) and Translocation Factor (TF) values. According to Yoon et al. BCF is the ratio of metal concentration in plant tissues to the metal concentration in the growth medium used to describe the plant's ability to accumulate metals. BCF = Heavy metals in plant . gkg !" ) Heavy metals in soil . gkg !" ) Meanwhile. TF is the ratio of metal concentration in the shoot to the root, which is used to indicate the plant's ability to translocate metals from the root to the upper part of the plant. Heavy metals in leaves . gkg !" ) TF = Heavy metals in roots . gkg !" ) The removal efficiency (RE) value was calculated using the equation proposed by Ren et al. This calculation aims to determine the percentage decrease in the concentration of the observed parameter after the treatment process. In the equation. Co is the initial concentration of the parameter in the wastewater . g LAA), while Ct is the concentration of the parameter after treatment . g LAA). The higher the RE value obtained, the more effective the treatment process is in reducing the concentration of the analyzed pollutant. The equation used to calculate the RE value is as follows: The RE value (%) is calculated using the equation proposed by Ren et al. as follows: RE =(Co-C. /Co x 100% Results and Discussion 1 Physiological Response and Biomass Plants will develop self-defense mechanisms so that physiological processes within them continue to function normally. The response of test plants to heavy metal stress during the study is presented in Figure 2. These physical changes only occurred in the first week, marked by the leaves of the plants starting to yellow and eventually drying out. Chlorosis can occur due to an increase in the production of Reactive Oxygen Species (ROS) triggered by oxidative stress in plants (Batra, 2. Exposure to lead (P. can disrupt the redox balance within plant cells through interference with physiological processes, such as photosynthesis and respiration, thereby causing ROS formation to exceed the capacity of the plantAos antioxidant defense system. Excessive accumulation of ROS triggers oxidative stress that can damage cellular components, such as lipid membranes, proteins. DNA, chlorophyll pigments, and cell organelles such as chloroplasts (Kiran et al. , 2. The damage can reduce chlorophyll content, triggering chlorosis symptoms characterized by the leaves turning yellow. Although there are physical changes in both types of plants, the application of Pb above standard limits does not affect the survival of T. angustifolia and C. alternifolius used in the study, as indicated by the plants' ability to continue producing shoots or offshoots. Table 2 shows that increasing Pb concentration does not have a significant effect on the dry weight biomass of both plant species. Based on these results, it can be stated that Pb exposure does not have a significant effect on plant biomass during the study period. The study results of Tang et al. also indicate that T. angustifolia is a potential adsorbent for removing Cd and Pb from aqueous solutions, where increasing Pb concentration and contact time do not have a significant effect on the decrease in plant biomass. In general, high biomass reflects the ability of plants to maintain growth while reducing the phytotoxic effects of heavy Figure 3. Growth of T. angustifolia (A) and C. alternifolius (B) under Pb stress Table 2. Plant biomass at the end of observation Treatments Tanpa Tanaman Dry Weight Biomass . Title Root 70 A 5. 77 A 1. 00 A 1. 33 A 6. 00 A 2. 83 A 4. Total Pb mg. L-1 Total Note: Letters that are the same are not statistically significantly different between treatments according to the DMRT 5% test, total data . ean A SD, n = . Heavy metals such as Pb. Cd, and Cr can cause oxidative stress through the formation of reactive oxygen species (ROS) that have the potential to damage proteins, lipids, and DNA of plant cells (Alam et al. , 2. To cope with these conditions, plants develop antioxidant defense systems in the form of enzymes such as SOD. POD. CAT. APX, and GPX that play a role in heavy metal detoxification, thereby increasing plant tolerance to Pb stress (Bah et al. , 2. This adaptive response is also observed in T. angustifolia treated with 20 mg Pb LAA, where root biomass . is higher than shoot biomass . This condition indicates a change in biomass allocation to the root system as an adaptation mechanism to heavy metal stress. Roots are the first organ to interact with Pb and serve as the main site for metal accumulation and detoxification, so plants tend to maintain root growth to support water and nutrient absorption while limiting Pb translocation to the shoot parts (Li et al. , 2. Although there are changes in biomass distribution between roots and shoots, the total biomass of T. angustifolia does not differ significantly among treatments, indicating that the plants are still able to maintain their growth and survival under Pb-exposed conditions. 2 Measurement Results of Temperature and pH in Artificial Wetlands The water temperature during the study ranged from 26Ae31AC, which is still within the optimal range for the phytoremediation process in constructed wetland systems. Chen et al. reported that a temperature of 25Ae30AC is a good condition to support the removal of heavy metals in artificial wetlands, while Truu et al. stated that a temperature range of 15Ae30AC is capable of supporting plant and microorganism metabolic activities. In C. alternifolius, a temperature range of 20Ae28AC has been reported to support enzymatic activity and minimize physiological stress, thus potentially increasing phytoremediation efficiency (Jinzhao et al. , 2. In the Typha genus, environmental conditions are also known to affect metal accumulation ability. Kola et al. reported that Typha capensis shows higher metal accumulation in the dry-winter and dry-summer seasons compared to the wet-summer The findings indicate that environmental factors, including temperature and hydrological conditions, play a role in regulating the processes of metal uptake and accumulation by Typha plants. The changes in temperature and pH are suspected to be related to processes occurring within the constructed wetland system. The decrease in water temperature is likely influenced by the presence of vegetation, which provides a shading effect on the water surface, thereby reducing solar radiation absorption (Kalny et al. , 2. Additionally, the evapotranspiration process of plants and heat exchange between water and soil media also play a role in maintaining the temperature stability of the system. Meanwhile, the increase in water pH is suspected to occur due to the photosynthetic activity of plants and microorganisms that utilize dissolved COCC in the water (Ghermandi et al. , 2. The reduction in COCC concentration leads to decreased formation of carbonic acid, causing a decline in HA ion concentration and an increase in water pH (Fonseca et al. , 2. These conditions indicate that the interaction between plants, soil media, and microorganisms contributes to creating a more stable environment during the treatment process. Changes in temperature and pH also affect the behavior of Pb in the system. Temperatures within the optimal range support the biological activity of plants and microorganisms so that the remediation process can proceed effectively. On the other hand, an increase in pH toward neutral can reduce the solubility and mobility of Pb in water, making the metal more easily adsorbed by soil media, precipitated as less soluble compounds, or bound to organic matter. According to Sukoasih et al. , temperature and pH are important factors that influence the solubility and bioavailability of heavy metals in aquatic Therefore, the temperature and pH conditions formed during the study are suspected to also support the high Pb removal efficiency in the constructed wetland system, reaching 85. 40Ae95. Table 3. Water temperature, water pH and soil pH on 1 DAP and 14 DAP Treatments Kontrol (Without Plant. Average Total Pb mg L-1 Water Temperature (AC) 1 DAP 14 DAP pH Water 1 DAP 14 DAP pH Soil 14 DAP 3 Pb Concentration in Water. Soil, and Plants after Remediation The presence of the heavy metal Pb in water has become a concern due to its persistent nature and difficulty in undergoing natural degradation. Heavy metals generally tend to bind with suspended particles, settle at the bottom of water bodies, and accumulate in solid phases such as sediments or aquatic organisms (Garvano et al. , 2. Based on Table 4. Pb levels in water decreased from the total Pb concentration added during the study, with Removal Efficiency (RE) values ranging from 85. 40Ae The high percentage of removal across all treatments indicates that the constructed wetland system is capable of effectively reducing Pb concentrations. Nevertheless. Pb concentrations in water did not significantly differ among treatments, suggesting that the presence of plants has not yet become the primary mechanism in the Pb removal process during the initial stage of remediation. The accumulation of Pb in the soil medium indicates that the process of metal sequestration occurs more through retention mechanisms by the soil rather than direct absorption by plants. Pb concentrations in the soil after remediation in the control treatment ranged from 3. 53Ae4. 04 mg kgAA, while in the T. angustifolia treatment they ranged from 1. 44Ae2. 20 mg kgAA, and in C. alternifolius from 0. 77Ae1. 54 mg kgAA (Table . This condition indicates that most Pb is initially retained in the medium, resulting in a decrease in metal concentration in the solution. This retention can occur through various mechanisms, such as adsorption on soil particle surfaces, cation exchange, complex formation with organic matter, and precipitation, which reduce the mobility and bioavailability of Pb (Liu et al. , 2022. Xing et al. Initial soil analysis showed that the medium has a sandy clay texture, pH 5. lightly acidi. , 4% organic carbon, and a CEC of 24. 59 cmol kgAA, which is considered medium. These characteristics support the ability of the media to retain Pb through interactions with clay fractions and organic matter, although the relatively high sand content still allows for the movement of metals in the soil solution (Nascimento et al. , 2. In addition, pH, organic matter, and CEC play an important role in controlling adsorption and the bioavailability of Pb for plant uptake (Georgin et al. , 2. Therefore, the high efficiency of Pb removal in constructed wetland systems is suspected to be not only caused by plant absorption but also by Pb retention in the soil media. Table 4. Pb Concentration in water after remediation Removal Efficiency (%) 21 A 0. Soil/ Control (Without plant. 20 A 0. 30 A 0. 28 A 0. 25 A 0. 35 A 0. 46 A 0. 43 A 0. 48 A 0. Description: Letters that are the same are not significantly different between treatments according to the DMRT 5% test, total data . ean A SD, n = . , tn . ot significantly affecte. RE (Removal Efficienc. Treatments Total mg Pb L-1 Pb in Water . g L-. Table 5 shows that administering different Pb concentrations to both plant species resulted in Pb absorption in the shoot and root parts that did not differ significantly. The highest Pb accumulation in shoots and roots was found in the T. angustifolia treatment at an initial concentration of 10 mg Pb LAA, followed by concentrations of 20 and 30 mg Pb LAA. Pb absorption by C. alternifolius in shoot and root tissues showed lower values compared to T. angustifolia, with Pb content in shoots ranging from 0. 47Ae 77 mg kgAA and in roots ranging from 0. 31Ae0. 61 mg kgAA. Although Pb removal efficiency reached 40Ae95. Pb accumulation in plant tissues was relatively low and did not show significant differences between treatments. These results are consistent with the high Pb accumulation still found in the soil medium, indicating that most Pb remained bound in the medium before being absorbed by the These results are consistent with the high accumulation of Pb still found in the soil media, indicating that most of the Pb is retained in the media before being absorbed by the plants. The presence of Pb in the roots and shoots shows that both species still play a role in the phytoremediation process. Heavy metals absorbed through the roots can be translocated to the shoots, where the metals are stored or detoxified as a mechanism of plant tolerance to heavy metal stress (Gupta et al. , 2. In general. Pb is a metal with low mobility, so its accumulation tends to be higher in the roots compared to the upper parts of the plant. However, in this study, the concentration of Pb in the shoots tended to be higher than in the roots, although the difference was not significant. This condition indicates that some of the Pb absorbed by the roots can be translocated to the above-ground tissues through the xylem flow influenced by the transpiration process. This translocation process is suspected to involve metal transporter systems, such as Heavy Metal ATPases (HMA) and the ZIP family proteins, which play a role in the movement of metals from roots to shoots (Gupta et al. , 2. The presence of Pb in shoot tissues shows that both species have the ability to distribute metals to the upper parts of the plant as one of the mechanisms for tolerance and detoxification against heavy metal stress. Table 5. Pb uptake by T. angustifolia and C. Pb in Soil Pb in Plant . g kg-. Total Pb mg L-1 Treatments . g kg ) Shoots Root Soil/ Control 04 A 0. (Without 75 A 0. 53 A 0. 20 A 0. 45 A 0. 35 A 0. 69 A 0. 25 A 0. 01 A 0. 44 A 0. 93 A 0. 72 A 0. 54 A 0. 77 A 0. 61 A 0. 26 A 0. 65 A 0. 50 A 0. 77 A 0. 47 A 0. 31 A 0. Note: Letters that are the same are not statistically significantly different between treatments according to the 5% DMRT test, total data . ean A SD, n = . 4 Bioconcentration Factor and Translocation Factor Values The ability of plants to accumulate heavy metals can be seen by looking for the Bioconcentration Factor (BCF) and Translocation Factor (TF) values presented in Table 6. Table 6 shows that T. angustifolia treatment at each concentration has BCF > 1 and TF > 1. Meanwhile. alternifolius has BCF < 1 but TF > 1. According to Sugiyanto et al. , there are 3 categories of plant groups related to BCF and TF values, namely BCF > 1 including accumulator plants. BCF < 1 including excluder plants. BCF close to 1 including accumulator indicator plants. TF > 1 for phytoextraction, and TF < 1 for Table 6. BCF and TF values Treatments Total Pb mg. L-1 BCF Plants with TF > 1 indicate that the plants are part of the phytoextraction mechanism, so the aerial parts of the plant above the ground can be easily harvested. High Pb accumulation can be associated with a good plant detoxification mechanism based on sequestration . of heavy metal ions in vacuoles, by binding them in the form of complex compounds with organic acids, proteins, and peptides (Cui et , 2. According to Nascimento et al. , the translocation process is generally influenced by mechanisms, root and leaf transpiration, as well as the solubility of metals in the soil, which is restricted due to adsorption to soil mineral fraction particles. According to Tangahu et al. , phytoextraction is the uptake or absorption and translocation of heavy metals by plant roots to the above-ground parts of the plant . that can be harvested and burned to obtain energy while recycling metals from the ash. Based on the statement of Soda et al. , wetland plants with high biomass and high TF allow the above-ground parts of the plant to be easily harvested without requiring much effort. New shoots can also grow from the remaining roots. However. Wang et al. stated that the TF value in wetland systems using emergent plants is not very important as an indicator of plants acting as phytoremediators because the root and shoot parts can be easily harvested. Conclusion The plants T. angustifolia and C. alternifolius have been proven capable of reducing lead (P. levels using the Free Water Surface Flow Constructed Wetland method in batch type, with Pb reduction over 2 weeks reaching 85-95%. Treatment with T. angustifolia at each given Pb concentration had BCF > 1 and TF > 1, whereas for C. BCF < 1 and TF > 1. Both plants are considered species of heavy metal hyperaccumulators with potential in practical applications for cleaning environments contaminated with heavy metals and have potential as phytoremediation with TF values > 1. Authors Note The authors declare that there is no conflict of interest regarding the publication of this article. Authors confirmed that the paper was free of plagiarism. Reference