BioLink : Jurnal Biologi Lingkungan.
Industri dan Kesehatan.
Vol.
August .
ISSN: 2356- 458X .
ISSN: 2550-1305 .
DOI: 10.
31289/biolink.
BioLink Jurnal Biologi Lingkungan.
Industri.
Kesehatan Available online http://ojs.
id/index.
php/biolink IDENTIFICATION OF SECONDARY METABOLIC AND TEST OF ACTIVITY ETHYL ACETATE FRACTION OF BANGUN- BANGUN (COLEUS AMBOINICUS LOUR.
) LEAVES AS ANTIOXIDANT
Kasta Gurning Departement Pharmacy.
Sekolah Tinggi Ilmu Kesehatan Senior Medan.
Indonesia Submited : 13-02-2020.
Accepted : 21-04-2020: Published : 10-08-2020 *Corresponding author: E-mail: kastagurning@gmail.
Abstract Antioxidants have an important role in delaying the oxidation process and preventing the occurrence of degenerative diseases in the body.
Antioxidants consist of synthetic and natural.
the use of synthetic antioxidants in a relatively long period is hazardous so that the use of natural antioxidants is considered more potential and safe for the body.
The process of extracting secondary metabolites from the leaves of bangun-bangun (Coleus amboinicus L.
) is carried out by the maceration method with methanol, then partitioned in stages with n-hexane, chloroform, and ethyl acetate.
This research intends to identify secondary metabolites and test antioxidant activity from the ethyl acetate fraction with the DPPH Phytochemical screening results of ethyl acetate fraction showed the different secondary metabolite groups such as alkaloids, phenolics & polyphenols, flavonoids, coumarin, and triterpenoids.
Antioxidant activity (IC.
obtained is 64.
97 with a healthy category, so that the potential to be used as a natural antioxidant.
Keywords: Antioxidants.
Phytochemical Screening.
Bangun-bangun Leaves, and IC50 How to Cite: Gurning.
Identification of secondary metabolic and test of activity ethyl acetate fraction of bangun- bangun (Coleus amboinicus lour.
) Leaves as antioxidant.
BioLink: Jurnal Biologi Lingkungan.
Industri dan Kesehatan.
Vol.
: Hal.
Gurning.
Identification of secondary metabolic and test of activity ethyl acetate fraction of bangun- bangun (Coleus amboinicus lour.
) Leaves as antioxidant INTRODUCTION diterpenoid, triterpenoid, sesquiterpenoid.
Antioxidants have an essential part phenolic, flavonoid, alcohol, and aldehyde in delaying the process of oxidation in esters (Arumugam et al.
, 2016.
Riyanto et food and preventing degenerative diseases al, 2.
which has antioxidant activity, for health caused by free radicals (Rani, diarrhea, flatulence, constipation, cough Akbarirad Neurodegenerative liver-kidney, include coronary heart disease, stroke, cytotoxic, anti-inflammatory, anti-diabetic, diabetes, and cancer (Serlahwaty et al.
analgesic, immunomodulatory, stimulate The free radicals are unstable breast milk production .
and atomic/molecular species because they hepatoprotective (Damanik et al.
, 2.
have one or more unpaired electrons (Mbah et al.
, 2.
Minimizing the MATERIALS AND METHODS influence of free radicals requires an Preparation Antioxidants can be divided amboinicus Lour.
into two, such as synthetic and natural.
Coleus Fresh leaves samples were taken and The use of synthetic antioxidants is Sub-district hazardous for health because it has side Parmaksian (Toba Samosir distric.
The The side effects caused by using sample is cleaned and dried in an open synthetic antioxidants in a long period room, which avoids direct contact with such as liver swelling, affecting enzymes.
The sample is pulverized by fatal bleeding in the pleural cavity, and using a blender.
the powder is continued drops of the epididymis and pancreas for extraction.
This research was carried (Andarwulan et al.
, 1.
The use of out in the Pharmacy laboratory at STIKes natural antioxidants is safer and beneficial Senior Medan in January-February 2020.
for health and has much lower toxicity (Biswas et al.
, 2.
Preparation and extraction process of Bangun-bangun leaves have a variety Coleus amboinicus Lour.
of bioactive compounds that can be tested 950 g of Coleus amboinicus Lour.
for their activity.
Coleus amboinicus L.
Simplicia 76 volatile compounds and 30 non-volatile methanol .
for 2 days.
After 2 days, it compounds such as the monoterpenoid, was filtered using Whatman No.
1 paper.
BioLink : Jurnal Biologi Lingkungan.
Industri dan Kesehatan.
Vol.
August .
: hal.
Then the filtrate was concentrated using a methanol 515 nm (Kedare & Singh, 2.
vacuum rotary evaporator to obtain a crude extract, and the residue was two Variations concentration of ethyl acetate fraction Coleus amboinicus Lour.
, which continued by partitioning gradually using used 10 ppm, 20 ppm, 30 ppm, 40 ppm, n-hexane .
, chloroform .
, and ethyl and 50 ppm and methanol with DPPH as acetate solvents.
The ethyl acetate fraction negative control and ascorbic acid with was concentrated, and a viscous extract variations in the concentration of 2.
5 ppm, was obtained.
The viscous ethyl acetate 0 ppm, 3.
5 ppm, 4.
0 ppm, and 4.
5 ppm.
extract was continued to phytochemical All of the measurements were carried out screening and testing its antioxidant with three times replication.
Calculation % inhibition against a reduction of free Screening The acetate fraction UV-Vis following equation (Gul et al.
, 2.
Secondary metabolite compounds Where abs = absorbance.
Abs control standard phytochemical methods (Kumar = total radical activity without the sample et al.
, 2013.
Gul et al.
, 2.
and abs test sample = activity in the Test of antioxidant activity with the presence of the test composition.
IC50 of DPPH method the linear regression equation y = bx a.
Antioxidant activity of the ethyl acetate fraction is carried out in vitro.
2,2,
RESULTS AND DISCUSSION
diphenyl-1-picrylhydrazyl (DPPH) is used Preliminary Phytochemical Screening The weight of ethyl acetate fraction is as a source of free radicals.
DPPH was dissolved with methanol .
at a Coleus amboinicus Lour.
, obtained by concentration of 0.
4 mM and incubated for 43A0.
02 g.
Results of phytochemical screening ethyl acetate fraction showed Absorbance DPPH compounds (Table .
Gurning.
Identification of secondary metabolic and test of activity ethyl acetate fraction of bangun- bangun (Coleus amboinicus lour.
) Leaves as antioxidant Table 1.
Secondary screening metabolites of ethyl acetate Coleus amboinicus Lour.
Secondary Metabolites Alkaloids Flavonoids Phenolic and Coumarin Triterpenoids Steroids Reagent ethyl acetate Mayer Dragenddroff Wagner Shinoda test FeCl3 5% in ethanol KOH 5% in ethanol Liebermann Bouchard Antioxidants activity test of concentration variation in each 5 mL Scavenging activity of free radical Coleus concentration label.
The extract flask is amboinicus Lour.
Is testing by the DPPH added 1 mL DPPH 0.
4 mL, and methanol is Ascorbic acid is used as a positive added to the 5 mL limit mark and then control and methanol as a solvent.
The incubated for 30 minutes.
The absorbance high value of antioxidants that support measurements were performed at a assert as inhibition concentrations of 50 maximum wavelength of 515 nm with a (IC.
The short of measuring antioxidant UV-Vis spectrophotometer (Table 2 and activity was carried out by adding 250 L Table .
Table 2.
Measurement of antioxidant activity of ethyl acetate fraction by DPPH method [ ] ppm Absorbance Inhibition (%) Average i i Blanko Table 3.
Measurement of antioxidant activity of ascorbic acid by DPPH method [ ] ppm Absorbance Inhibition (%) Average i i Blanko The measurement results for each proportional to the increased ability to concentration variations showed that the inhibit free radicals from DPPH.
The increase in concentration was directly higher the concentration of the fraction BioLink : Jurnal Biologi Lingkungan.
Industri dan Kesehatan.
Vol.
August .
: hal.
and assets of Coleus amboinicus Lour.
, the higher its ability to inhibit free radicals.
Figure 1.
Antioxidant activity of .
ethyl acetate Coleus amboinicus Lour fraction and .
ascorbic acid The IC50 coumarin, and triterpenoid.
It shows regression y= 0.
7638x 0,378.
R2= 0,9771
antioxidant activity with IC50 64.
97 with a in Figure 1a and 1b was 64.
97 with a healthy category.
strong category and antioxidant activity of ascorbic acid as a positive control with linear regression y = 18.
48x - 23.
RA =
9969 obtained IC50 value of 3.
97 with a very strong category (Nihlati et al.
, 2.
The ability of strong antioxidant activity of ethyl acetate Coleus amboinicus Lour.
fraction due to the presence of active compounds of phenolic & polyphenol and flavonoid groups.
Phenolic & polyphenol groups and flavonoids are reported to be responsible as strong antioxidants in every natural ingredient (Skrovankova et , 2.
CONCLUSION
The Coleus amboinicus Lour fraction has a variety of .
lkaloids, flavonoids, phenolic and polyphenols.
REFERENCES