Jurnal Pengabdian Kepada Masyrakat, e-ISSN: 2775-2437 Vol.
1 No.
1 Edisi Juni 2021 https://ejournal.
id/index.
php/JPK =============================================================================================== Received: 14 June 2021 :: Accepted: 16 June 2021 :: Published: 30 June 2021
SOCIALIZATION OF ANALYSIS OF TOTAL TERPENOIDS FROM
MANILTOA GRANDIFLORA (A.
GRAY) SCHEFF LEAVES
USING TLC METHODS
Jhon Patar Sinurat1*.
Reh Malem br Karo2.
Rinaldo Berutu3 Departement of Medical Laboratory Technology.
Institut Kesehatan Medistra Lubuk Pakam Departement of Pharmacy.
Universitas Prima Indonesia Departement of Pharmacy.
Institut Kesehatan Medistra Lubuk Pakam Sudirman street No.
Deli Serdang.
North Sumatera, 20512.
Indonesia Jln.
Sudirman No.
38 Lubuk Pakam.
Kabupaten Deli Serdang.
Sumatera Utara Ae Indonesia *email korespondensi author: jhonpatar12@gmail.
DOI 10.
35451/jpk.
Abstract Saputangan leaves contained terpenoids which appeared reddish brown rings when tested using Liebermann Burchard.
Salkowski and cerric sulfate.
Saputangan leaves were macerated, then partitioned to obtain total terpenoids using a separatory funnel while checking filtrate partition with cerric sulfate.
Extracts dissolved with methanol were partitioned with n-Hexane and then partitioned between aquadest and ethyl acetate in a ratio of 1: 1 to obtain 50 g of total terpenoids.
TLC analysis was carried out on total terpenoids using n-hexane: acetone .
:20 v/.
as the mobile phase and TLC plate with silica gel surface.
The elution process was carried out in the chamber and the results of TLC were sprayed using Cerric Sulfate reagent.
The total terpenoids were proved that terpenoids have 11 terpenoids as red stain based on their Rf.
Based on the results of tests conducted on participants who were involved in socialization, it was stated that around 98% of participants had understood about terpenoid compounds, the extraction process of these compounds and how the elution process in TLC was.
After socializing the material about these terpenoid compounds, the public increasingly understands that there are many chemical compounds that are beneficial to human health whose sources come from plants.
These chemical compounds can be secondary or primary metabolites.
Keywords: Terpenoids.
Maceration.
Partition.
TLC and Rf Introduction Terpenoids could act as important material in production of flavors, fragrances, high grade lubricants, biofuels, agricultural chemicals, and Most of the terpenoids are used for the treatment of many Terpenoids component in atsiri oil.
Sesquiterpenoids Additionally, -terpene terpinolene in Melaleuca alternifolia could contributed in antioxidant activity.
Some terpenes are potent against heart disease, malaria, and cancer.
(Rassem et al.
, 2.
Terpenes consist five carbon as isoprene units which are connected to each other by thousands of ways.
Terpenoids are divided into diterpenes,sesterpenes, and triterpenes.
(Perveen, 2.
Terpenes are simple hydrocarbons, with non-polar propeties (Lingala & Ghany, 2.
Saputangan (Maniltoa grandiflora (A.
Gra.
Schef.
are plant that included to the genus Maniltoa and Fabaceae family that was mostly useful as medicinal.
Parts used as medicine are leaves, flowers, root bark, and bark.
Jurnal Pengabdian Kepada Masyrakat, e-ISSN: 2775-2437 Vol.
1 No.
1 Edisi Juni 2021 https://ejournal.
id/index.
php/JPK =============================================================================================== Received: 14 June 2021 :: Accepted: 16 June 2021 :: Published: 30 June 2021 Saputangan leaves according to the screening test was positively contained Terpenoids.
Saputangan plants are usually used as ornamental plants that can reduce pollution by absorbing pollutants such as carbon monoxide.
(Scheff & Sinurat, 2.
Terpenoids maceration and partition.
Maceration is immersing plants .
n the form of powde.
in a closed container with the solvent at room temperature, which is allowed to soak for a certain period with several times stirring.
(Nn, 2.
Partition is using two immiscible solvents in a separating funnel, and the compounds are distributed in two solvents according to their different partition coefficients.
(Otsuka, 2.
Analysis of total terpenoid is Layer Chromatography (TLC).
Thin layer chromatography can be used to separating the compounds present in a mixture and determining the purity of a TLC is performed on an adsorbent material, usually silica gel, alumina or cellulose.
(Namir et al.
This seminar aims to socialize and educate the member of seminar, so that understand that terpenoid compounds are secondary metabolites that are very beneficial for human health.
The socialization of the saputangan plant was also explained in full starting from phytochemical screening to identify terpenoid compounds, extraction using maceration and partition methods in obtaining plant extracts and conducting TLC analysis to see the content of terpenoid compounds in saputangan.
procedure steps used in this service activity are as follows:
Preparation stage Prepare containing the equipment and materials used as well as media that support this The samples used were saputangan leaves which were prepared in powder form.
Materials: Saputangan leaves powder.
Ethanol.
Methanol.
Ethyl Acetate, nHexane.
Acetone.
Reagent of Cerric Sulfate.
Reagent of Liebermann Burchard.
Reagent of Salkowski and Aquadest.
All the solvents and reagents used in this study as pro analysis and High grade.
Equipments: Macerator and Separatory Funnel Schoot Duran merck.
Waterbath Memert merck.
Rotary Evaporator Heidolph Merck.
TLC performed using silica gel 60F254 (E.
Merc.
and Chamber.
Methods This service activity is carried out through seminars using the lecture method, question and answer, and Identification terpenoid compounds in Saputangan phytochemical screening.
Extraction of saputangan leaves was carried out by maceration and partition methods.
TLC
analysis was carried out to analyze the terpenoid total in the sample.
The Evaluation and Follow-up Implementers of service activities evaluate the understanding of seminar participants through discussions and questions and answers regarding the results of TLC analysis of terpenoid Then carry out follow-up activities when participants are still not able to understand well.
Implementation Stage Socialization TLC analyses of Terpenoid was carried out directly to participants through seminar This socialization is expected to open and broaden the participants The leaf extract obtained by maceration with etanol and partition with ethyl acetate and n-hexane.
The terpenoid reagents used were cerium Burchard.
Thin layer chromatography analysis was carried out on dry extract using a stationary phase of Merck 60F254 silica gel which was found on the surface of the TLC plate.
Measure of TLC plat is 10 x 2 cm.
The mobile phase was used n-hexane : acetone with a ratio solvents varying 90:10, 80:20, 70:30 and 60:40 v/v.
Jurnal Pengabdian Kepada Masyrakat, e-ISSN: 2775-2437 Vol.
1 No.
1 Edisi Juni 2021 https://ejournal.
id/index.
php/JPK =============================================================================================== Received: 14 June 2021 :: Accepted: 16 June 2021 :: Published: 30 June 2021 Result and Discussion Terpenoids screening The terpenoids in sample were analysed use Cerric Sulfat.
Liebermann Burchard and Salkowski Reagents.
The results in Figure 1 showed Maniltoa grandiflora contained terpenoids with the appearance of a reddish brown ring on the sample.
(Malik et al.
, 2.
The results obtained from this service activity are as follows:
The participants as evidenced by the result of pre-test and post-test Seminar knowledge about the benefits of several ornamental plants that can Socialization showed in figure 3.
Figure 1.
Terpenoid screening Maceration and Partition Macerate evaporated using a rotary evaporator to obtain a solid extract of 350 g.
Partition was used n-hexane and ethyl acetate which are carried out in stages so that the extract of the saputangan leaves is contained a total terpenoid as 50 g.
Figure 3.
Socialization by application Conclusion Saputangan leaves terpenoids which appeared reddish Liebermann Burchard.
Salkowski and cerric sulfate.
Saputangan leaves were macerated, then partitioned to obtain total terpenoids using a separatory funnel while checking filtrate partition with cerric sulfate.
The total terpenoids were analyzed with TLC Methods and proved that terpenoids have 11 terpenoids as red stain based on their Rf.
Based on the results of tests conducted on participants involved in socialization, it was stated that around 98% of participants had understood terpenoid compounds.
TLC Analyses of Total Terpenoid TLC analyses was performed with TLC using n-Hexane: Acetone .
:20 v/.
as elunet.
After the elution, the TLC plate was sprayed with Cerric Sulfate reagent and heating due to all terpenoids and steroids (Ludwiczuk et , 2.
will produce red stain.
Stains were separated into 11 stains which had a different retention factor (R.
There were Rf1 .
Rf2 .
Rf3 .
Rf4 .
Rf5 .
Rf6 .
Rf7 .
Rf8 .
Rf9 .
Rf10 .
dan Rf11 .
which are shown in figure 2.
Figure 2.
Total Terpenoid Acknowledgement Authors sincerely appreciate the financial and moral support from:
Direktorat Penelitian & Pengabdian Masyarakat
(DRPM),
Kementrian Riset.
Teknologi, & Pendidikan Tinggi Republik Indonesia.
Institut Kesehatan Medistra Lubuk Pakam.
TLC
Jurnal Pengabdian Kepada Masyrakat, e-ISSN: 2775-2437 Vol.
1 No.
1 Edisi Juni 2021 https://ejournal.
id/index.
php/JPK =============================================================================================== Received: 14 June 2021 :: Accepted: 16 June 2021 :: Published: 30 June 2021
References