ISSN 2808-3229 Journal of Biodiversity and Biotechnology. , 86Ae94, 2024 URL: https://jurnal. id/jbb/article/view/109410 doi: http://dx. org/10. 20961/jbb. Effect of Watering Frequency and Organic Fertilizer on The Growth and Yield of Ground Nut Berliana Nur Rizkyah1*. Supriyono1. Maria Theresia Sri Budiastuti1. Desy Setyaningrum2 Department of Agrotechnology. Faculty of Agriculture. Sebelas Maret University. Surakarta. Indonesia Diploma in Agribusiness. Vocational School. Sebelas Maret University. Surakarta. Indonesia *Corresponding Author: berliananurrizkyah@student. Abstract Groundnut (Arachis hypogae. is one of the commodities with high economic value. however, weather anomalies can affect plant water availability, which in turn impacts groundnut production and If water availability is not balanced with proper fertilizer application, plant growth may be inhibited. This study aims to determine the role of watering frequency and organic fertilizer on the development and yield of groundnut. The research was conducted at the Laboratory of the Faculty of Agriculture. Sebelas Maret University, located in Sukosari Village. Jumantono District. Karanganyar Regency, over a period of four months from December 2024 to March 2025. The method used was a factorial Randomized Complete Block Design (RCBD), consisting of two factors and three replications. The first factor was watering frequency . nce a day, once every two days, once every three days, and once every four day. The second factor is the organic fertilizer dosage . , 10, 20, and 30 tons ha-. Data were analyzed using Analysis of Variance (ANOVA) at a 95% significance level, followed by DuncanAos Multiple Range Test (DMRT) at a 95% significance level. Regression analysis was performed to determine the recommended dosage, and correlation analysis was used to identify relationships between observed variables. The result showed that watering once every three days resulted in the best 100-seed weight. Watering once a day resulted in the best dry biomass weight. Organic fertilizer dose of 20 t. ha-1 produced the best plant height, leaf area, leaf area index, number of pods, fresh pod weight, dry pod weight, number of seeds, and seed weight. Organic fertilizer dose of 30 t. ha -1 produced the best number of leaves at 5 Weeks After Planting (WAP), number of nodes at 5 WAP, and flowering age. Interaction between watering once a day and an organic fertilizer dose of 20 t ha-1 produced the best dry biomass weight. Keywords: Alfisol. Leguminosae. nutrient uptake. slow release. Cite this as: Rizkyah. Supriyono. Budiastuti. Setyaningrum. Effect of Watering Frequency and Organic Fertilizer on The Growth and Yield of Ground Nut. Journal of Biodiversity and Biotechnology. , 80Ae94. doi: http://dx. org/10. 20961/jbb. Introduction Groundnut (Arachis hypogae. is one of the commodities with high economic value. In addition to its high nutritional content, the financial prospects of Groundnut are also considered very promising, as they can be processed into various products that add economic value in the food industry. This, in turn, can drive an increase in farmersAo income across different regions . The Indonesian population widely consumes groundnuts, and thus, the demand for groundnuts continues to rise each year in line with population growth. However, this increasing demand is not supported by a corresponding increase in production . Several factors, including climate change and insufficient nutrient availability, can cause the decline in Groundnut Water availability can be affected by weather anomalies, which may lead to either water scarcity or excess, thereby influencing the production and productivity of groundnut. Water stress can hinder the opening of leaf stomata, which in turn affects the plant's physiological processes and ultimately its yield . One of the factors contributing to the decline in peanut production is insufficient water availability during the growth process, which significantly impacts peanut yield . Water is the main component of active tissues, cell growth, and the maintenance of cell turgor . If water availability is not balanced with the proper application of fertilizers, plant growth will be inhibited . Water and fertilizer are two essential and interrelated factors that significantly influence plant growth. Water acts as the solvent, while fertilizer serves as the Water functions to dissolve and transport the nutrients contained in fertilizers, whereas fertilizers provide the essential nutrients plants need for growth, development, and production. Fertilization is a common alternative to support efforts in increasing peanut yield, especially in nutrient-deficient soils . Organic fertilizer has advantages because it not only improves soil fertility but also enhances the physical, chemical, and biological properties of the soil . The fertilizer used in this study is cow manure. Compared to other types of manure, cow manure contains a higher fiber content, which helps loosen the soil, improves its texture, facilitates plant growth, and provides both macro- and micronutrients. This study aims to determine the appropriate watering frequency and organic fertilizer dosage for the development and yield of Materials and Methods This study was conducted on Alfisol soil at the Faculty of Agriculture Laboratory. Sebelas Maret University (UNS), located in Sukosari Village. Jumantono Sub-district. Karanganyar Regency, for a duration of four months, from December 2024 to March 2025. The research site is situated at the coordinates 7A37Ao829Ay and 110A56Ao901Ay E. The initial soil analysis was conducted at the Agricultural Instrument Standardization Center (BSIP) in Yogyakarta. Data collection and measurement of results were conducted at the Laboratory of Ecology and Plant Production Management. Faculty of Agriculture. UNS. Tissue analysis was performed at the Laboratory of Soil Chemistry and Fertility. Faculty of Agriculture. UNS. The materials used in this study were groundnut seeds of the Kancil variety and cow manure fertilizer. The tools employed included 35 y 35 cm polybags, an oven, and an analytical The experimental design used was a factorial Randomized Complete Block Design (RCBD) with two factors and three replications. The first factor was watering frequency . nce every 1, 2, 3, and 4 day. The second factor was the application of organic fertilizer dosages . , 10, 20, and 30 tonsAh. The observed variables included plant height, number of leaves, number of nodes, flowering age, leaf area, leaf area index, fresh shoot weight, dry shoot weight, number of pods, fresh pod weight, dry pod weight, number of seeds, seed weight, weight of 100 seeds, as well as tissue analysis and NPK uptake of plants. Data were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level, followed by DuncanAos Multiple Range Test (DMRT) at a 95% confidence level. Regression analysis was employed to determine the recommended fertilizer dosage, and correlation analysis was conducted to investigate the relationships among the observed variables. Results and Discussion General Research Conditions Optimal environmental conditions can enhance the growth rate and yield of groundnut. The environmental factors that need to be considered include air temperature, humidity, and light intensity. The average temperature at the research site was 30. 9AC, with a maximum 4AC and a minimum of 27. 1 AC. The air humidity at the site was 68%, with the highest recorded humidity at 83% and the lowest at The average light intensity at the research site was approximately 11,660 lux, with the lowest recorded intensity at 4,960 lux and the highest at 22,440 lux. Based on the initial soil analysis, the soil used had a pH of 6. 85, which is classified as neutral. The organic C content was 0. categorized as low. The total N content was 70%, which is considered high. The available K content of the soil was 157 ppm, also classified as high. The PCCOCI content was six ppm, which falls into the low category. The C/N ratio of the soil was 2, which is considered very Alfisol soil is a type of soil with low fertility, making it less suitable to support agricultural activities . An effort to improve the nutrient content of the soil is the application of cow manure, which contains 1. 24% N, 41% PCCOCI, 1. 56% KCCO, 21. 21% organic C, a C/N ratio of 17. 03%, and a pH of 6. Table 1. Plant height, number of leaves, number of nodes, and flowering age of Groundnut under different watering frequencies and organic fertilizer dosages Flowering Age Plant Height Number of Leaves Number of Nodes Treatment (DAS) Watering Frequency . ays interva. 37,85A4,17 19,41A3,95 15,66A3,16 33,35A4,35 38,50A2,37 21,04A3,56 16,62A2,09 33,35A4,35 37,51A3,51 20,79A2,95 16,70A2,42 33,83A2,72 36,70A2,36 20,08A2,87 16,54A2,80 32,66A3,45 Organic Fertilizer Dosage . onAh. 34,24A2,76 17,33A2,71 13,95A2,08 35,58A3,60 37,97A2,74 20,87A3,16 16,75A2,64 32,66A3,66 39,19A2,59 21,45A3,17 16,87A1,72 33,25A3,17 39,17A1,75 21,66A2,50 17,95A2,24 31,50A3,45 Interaction Significance Watering Frequency 1,37 0,77 0,53 0,26 Organic Fertilizer Dosage 13,34 5,86 6,55 3,44 Interaction 1,75 0,38 0,46 1,06 Coefficient of Variation (%) 5,89 14,27 14,10 9,64 Notes: DAS = Days After Sowing. (A. indicates no interaction. Plant Height Based on the results of variance analysis (Table . , watering frequency had no significant effect on plant height, and there was no interaction between watering frequency and organic fertilizer application. The organic fertilizer dosage had a significant impact on groundnut yield at 5 weeks after planting (WAP). DuncanAos multiple range test showed that the application of 20 tonsAha of organic fertilizer resulted in a significantly greater plant height at 5 WAP compared to 0 tonsAha. stated that plants absorb the availability of soil nutrients and play a vital role in supporting different stages of plant growth and development. Number of Leaves Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of various organic fertilizer dosages had a highly significant impact on the number of peanut leaves at 5 weeks after planting (WAP). DuncanAos multiple range test showed that the application of 30 tonsAha of organic fertilizer resulted in a significantly higher number of leaves at 5 WAP compared to 0 tonsAha. stated that leaves require nitrogen (N), which plays an essential role in chlorophyll formation. Number of Nodes Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of organic fertilizer dosages had a highly significant impact on the number of groundnut nodes at 5 weeks after planting (WAP). DuncanAos multiple range test showed that the application of 30 tonsAha of organic fertilizer resulted in a significantly higher number of nodes at 5 WAP compared to 0 tonsAha. Organic fertilizer contains both macro- and micronutrients. According to . , macro- and micronutrients can increase plant height and the number of Flowering Age Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of various organic fertilizer dosages had a substantial impact on the flowering age of groundnut. DuncanAos multiple range test showed that the application of 30 tonsAha of organic fertilizer significantly affected the flowering age compared to 0 tonsAha. According to . , flowering age can be influenced by the sufficient availability of nutrients such as nitrogen, phosphorus, and potassium. Table 2. Leaf area, leaf area index, fresh shoot weight, and dry shoot weight of Groundnut under different watering frequencies and organic fertilizer dosages Fresh Biomass Dry Biomass Treatment Leaf Area . Leaf Area Index Weight . Weight . Watering Frequency . ays interva. 40,28A6,22 1,38A0,37 50,46A11,78 11,61A2,04 45,97A4,43 1,56A0,39 41,85A9,79 9,67A1,61 39,19A6,13 1,44A0,26 37,32A6,79 8,45A2,05 40,86A4,45 1,46A0,28 41,14A5,96 10,26A1,47 Organic Fertilizer Dosage . onAh. 40,43A3,44 1,11A0,16 40,21A7,65 10,01A1,97 41,19A3,80 1,52A0,28 42,12A10,58 10,43A1,68 42,95A5,31 1,63A0,33 44,29A12,26 10,56A2,25 46,33A5,98 1,58A0,24 44,15A9,17 10,45A1,91 Interaction Significance Watering Frequency 0,55 1,04 5,80 3,44 Organic Fertilizer Dosage 4,65 10,15 0,70 0,27 Interaction 0,42 0,24 1,73 2,53 Coefficient of Variation (%) 10,65 17,48 18,70 15,62 Notes: ( ) indicates interaction. (A. indicates no interaction. Leaf Area Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of various organic fertilizer dosages had a highly significant impact on the leaf area of groundnut. DuncanAos multiple range test showed that the application of 20 tonsAha of organic fertilizer resulted in a significantly larger leaf area compared to the application of 0 tonsAha. This indicates that the application of organic fertilizer increases the leaf area of groundnuts. According to . , nitrogen (N) plays a vital role in enhancing plant growth and yield, as well as producing wider and greener leaves. Leaf Area Index Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of various organic fertilizer dosages had a highly significant impact on the leaf area index of DuncanAos multiple range test showed that the application of 20 tonsAha of organic fertilizer resulted in a significantly higher leaf area index compared to the application of 0 tonsAha. Organic fertilizer contains NPK, which is essential for leaf According to . , nutrient availability influences the leaf area index as it affects leaf Dry Shoot Weight Based on the results of variance analysis (Table . , organic fertilizer application had no significant effect, whereas watering frequency had a significant impact on the dry shoot weight of groundnuts. The results of DuncanAos multiple range test showed that different watering frequencies had a substantial effect on dry shoot weight, with watering once a day being significantly different from the other watering frequencies. According to . , the high daytime temperatures and low humidity in the greenhouse can increase the transpiration rate. Daily watering can reduce transpiration by fulfilling water requirements, thereby enhancing photosynthesis and influencing plant dry weight. further stated that higher dry shoot weight indicates better plant growth and development. Based on the results of the variance analysis (Table . , there was an interaction between watering frequency and organic fertilizer dosage on the dry shoot weight of Both water and nutrients are essential for photosynthesis and physiological According to . , plant dry weight can increase as a result of physiological processes during growth. If one of these factors is insufficient, plant growth will be inhibited. also explained that sufficient water availability helps plant roots absorb nutrients more effectively. Table 3. Number of pods, fresh pod weight, and dry pod weight of Groundnut under different watering frequencies and organic fertilizer dosages Treatment Number of Pods . Fresh Pod Weight . Dry Pod Weight . Watering Frequency . ays interva. 9,58A3,29 11,7A4,26 9,65A3,25 9,25A2,09 11,05A2,82 10,01A2,45 9,08A3,78 12,21A5,82 11,39A4,85 9,75A2,45 11,49A3,32 10,03A2,59 Organic Fertilizer Dosage . onAh. 8,08A1,83 9,59A3,04 8,50A1,93 9,25A2,49 12,13A3,39 10,59A2,02 11,25A4,03 13,775A5,66 12,35A5,30 9,08A2,07 10,95A2,95 9,64A2,03 Interaction Significance Watering Frequency 0,17 0,24 0,78 Organic Fertilizer Dosage 3,30 3,17 3,52 Interaction 1,09 1,38 0,98 Coefficient of Variation (%) 26,88 29,76 29,21 Notes: (A. indicates no interaction. Number of Pods Based on the results of variance analysis (Table . , watering frequency had no significant effect, and no interaction was found between watering frequency and organic fertilizer application. However, the application of different organic fertilizer dosages had a substantial impact on the number of peanut The results of DuncanAos multiple range test showed that the application of organic fertilizer at a dosage of 20 tonsAha had a significantly different effect compared to the dosage of 0 tonsAha. This finding is supported by . , who stated that nitrogen (N) plays a role in the vegetative growth of plants, phosphorus (P) contributes to flower and fruit formation, and potassium (K) plays a role in improving fruit quality in plants. Fresh Pod Weight Based on the results of variance analysis (Table . , watering frequency had no significant effect, and no interaction was observed between watering frequency and organic fertilizer application. However, the application of different organic fertilizer dosages had a significant impact on fresh pod The result of DuncanAos multiple range test indicated that the application of organic fertilizer at a dosage of 20 was significantly different from the dosage of 0 tonsAha. According to . , the availability of nutrients influences pod formation. Adequate nutrient availability supports the photosynthesis process, and the assimilates produced are translocated to the pods, thereby increasing the fresh weight of groundnut pods. Dry Pod Weight Based on the results of variance analysis (Table . , watering frequency had no significant effect, and no interaction was found between watering frequency and organic fertilizer application. However, the application of different organic fertilizer dosages significantly affected the dry pod weight of The results of DuncanAos multiple range test showed that the application of organic fertilizer at a dosage of 20 tonsAha . was significantly different from the dosage of 0 tonsAha. According to Siahaan and . , the absence of nutrient application can inhibit seed formation and result in lower pod . stated that nutrient availability can improve the quality and yield of crops. Similarly, . explained that nutrient availability is closely related to pod formation and filling. Table 4. Number of seeds, seed weight, and weight of 100 seeds of Groundnut under different watering frequencies and organic fertilizer dosages Treatment Number of Seeds . Seed Weight . Weight of 100 Seeds . Watering Frequency . ays interva. 18,00A6,11 7,66A2,92 42,64A11,28ab 17,00A3,64 6,76A1,55 39,80A4,94b 17,66A7,22 8,175A3,53 46,04A8,05a 17,91A3,92 7,00A2,23 37,32A6,46b Organic Fertilizer Dosage . onAh. 15,66A3,47b 6,02A1,72b 37,08A7,84 17,91A3,96ab 7,53A1,45ab 43,09A8,13 21,00A7,69a 8,88A4,03a 40,5A6,52 16,00A3,49b 7,175A1,95ab 45,14A9,73 Interaction Significance Watering Frequency 0,11 0,86 3,04 Organic Fertilizer Dosage 3,34 2,93 2,62 Interaction 1,30 1,08 1,26 Coefficient of Variation (%) 26,28 32,20 17,97 Notes: (A. indicates no interaction. Number of Seeds Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of different organic fertilizer dosages had a substantial impact on the seed weight of DuncanAos multiple range test showed that the application of 20 tonsAha of organic fertilizer resulted in a significantly higher number of seeds compared to 0 tonsAha. According to . , organic fertilizer improves soil structure, making it easier for peanut pods to form, which provides a suitable environment for seed development. reported that nitrogen (N) is required in larger amounts during seed filling, and a deficiency in N reduces seed development. stated that nutrient deficiencies can hinder overall plant growth and development. Seed Weight Based on the results of variance analysis (Table . , watering frequency had no significant effect, and there was no interaction between watering frequency and organic fertilizer application. The application of different organic fertilizer dosages had a substantial impact on peanut seed weight. DuncanAos multiple range test showed that the application of 20 tonsAha of organic fertilizer resulted in a significantly higher seed weight compared to 0 tonsAha. According to . , nitrogen (N) plays a role in increasing peanut . reported that the availability of N and K can promote weight gain. Nitrogen absorbed by the plant serves as an essential building material for leaves and seeds, thereby increasing dry seed weight. stated that phosphorus (P) is essential for seed formation. a deficiency can cause empty pods and shriveled seeds. The application of 20 tonsAha produced the best seed weight, as excessive organic fertilizer can reduce plant productivity. noted that over-fertilization may lead to plant wilting. Weight of 100 Seeds Based on the results of variance analysis (Table . , organic fertilizer application had no significant effect, and no interaction was observed between watering frequency and organic fertilizer application. However, watering frequency had a significant impact on the weight of 100 peanut seeds. DuncanAos multiple range test showed that watering every 3 days resulted in a significantly higher weight of 100 seeds compared to watering frequencies at other intervals. This indicates that a moderate watering frequency, neither too frequent nor too infrequent, can increase the weight of 100 peanut seeds. According to . , plants require water, but excessive or insufficient watering can cause stress, negatively affecting growth and yield. reported that water deficiency reduces the weight of 100 seeds, as seeds may not fully develop and are smaller than usual. Plant Tissue NPK The results of tissue analysis for nitrogen (N) showed the highest N content in plants treated with 20 tonsAha of organic According to . , nitrogen can be lost from the soil through three pathways: leaching via drainage, volatilization, and plant The tissue analysis for phosphorus (P) revealed the highest P content in plants treated with 30 tons ha of organic fertilizer. reported that P is absorbed more by plants during the generative phase than during the vegetative phase. The tissue analysis for potassium (K) revealed the highest K content in plants treated with 10 tons ha of organic According to . , potassium is not absorbed extensively by plants due to its high mobility in the soil. Plant NPK Uptake The results of nutrient uptake analysis showed that nitrogen (N) uptake was highest in plants treated with 20 tonsAha of organic fertilizer and lowest in plants treated with 0 tonsAha. According to . , nitrogen in the soil is highly mobile, which can lead to significant losses and reduce plant uptake of nitrogen. Phosphorus (P) uptake was highest in plants treated with 30 tonsAha of organic fertilizer and lowest at 0 tonsAha. reported that plant P uptake can be influenced by N uptake, as absorbed nitrogen stimulates root growth, enabling more effective P absorption from the soil. emphasized that phosphorus cannot be substituted by other nutrients, making adequate P uptake crucial for plant growth. Potassium (K) uptake was highest in plants treated with 10 tonsAha of organic fertilizer and lowest at 0 tonsAha. stated that increased soil K availability improves root development and plant biomass, thereby enhancing K uptake by plants. Correlation Analysis The correlation coefficient is a numerical value that describes the strength and direction of the relationship between two Generally, it ranges from -1 to 1. A correlation value of 0 indicates no linear relationship between the two variables. Based on the correlation analysis, peanut plant height showed a powerful relationship with leaf area . = 0. and leaf area index . = 0. This suggests that taller plants tend to have larger leaf areas and higher leaf area indices. The number of peanut leaves had a robust correlation with the number of nodes . = 0. and leaf area index . = 0. , indicating that an increase in leaf number is associated with more nodes and a higher leaf area index. stated that the number of leaves is directly proportional to plant height, so taller plants develop more stem nodes, which results in more The number of peanut pods showed a robust correlation with fresh pod weight . = . , dry pod weight . = 0. , number of seeds . = 0. , and seed weight . = 0. This suggests that more pods are associated with higher fresh and dry pod weights, a greater number of seeds, and heavier seed weights. increase in pod number generally leads to higher fresh and dry pod weights. reported that larger pod size and shape influence higher yield weight. Additionally, a higher number of pods is usually accompanied by an increase in seed number. According to . , the number of pods affects pod weight because the number of pods influences the total weight. noted that seed weight is affected by seed size, which is determined during pod filling. Conclusion Watering every 4 days resulted in the highest fresh shoot weight, watering every 3 days produced the best weight of 100 seeds, and watering every day resulted in the highest dry shoot weight. An organic fertilizer dosage of 20 tonsAha produced the best results for plant height, leaf area, leaf area index, number of pods, fresh pod weight, dry pod weight, number of seeds, and seed weight. An organic fertilizer dosage of 30 tonsAha resulted in the highest number of leaves at 5 weeks after planting (WAP), number of nodes at 5 WAP, and optimal flowering age. The interaction between daily watering and an organic fertilizer dosage of 20 tonsAha produced the highest dry shoot References