urnal Ilmu Sosial dan Pendidikan (JISIP) Vol.
9 No.
3 Juli 2025 e-ISSN : 2656-6753, p-ISSN: 2598-9944 DOI: 10.
36312/jisip.
8794/http://ejournal.
org/index.
php/JISIP/index The Role of Pseudomonas Putida in Bioremediation: a Review Andi Tirta Angraeni1.
Nila Amelia2.
Yusminah Hala3 Program Pascasarjana Universitas Negeri Makassar Universitas Negeri Makassar Article Info Article history:
Received: 22 May 2025 Publish: 1 July 2025 Keywords:
Pseudomonas Putida.
Role Of P.
Putida.
Bioremediation.
Abstract Aerobic gram-negative bacteria Pseudomonas putida is commonly found in soil and air.
It is renowned for its extraordinary metabolic ability to break down various complex organic materials, such as aquaculture This article discusses the role of P.
putida in bioremediation and the ability of P.
putida to break down organic compounds such as aromatic and aliphatic chemicals.
This study identified key genetic traits and metabolic pathways that enhance the efficiency of bacterial bioremediation by synthesizing relevant literature using a descriptive qualitative approach.
These results demonstrate the importance of utilizing the diverse characteristics of bacteria for innovative bioprocesses that can produce sustainably manufactured products, which in turn promotes biotechnology-based economic development and environmental sustainability.
This is an open access article under the Lisensi Creative Commons Atribusi-BerbagiSerupa 4.
0 Internasional Corresponding Author:
Andi Tirta Angraeni Institusi: Universitas Negeri Makassar Email: anditirtaangraeni@gmail.
INTRODUCTION
Pseudomonas putida is an aerobic gram-negative bacterium commonly found in water and soil.
This bacterium has excellent metabolic capabilities, enabling it to decompose various complex organic compounds, including waste from fish farming activities.
11 P.
putida has received great attention over the last few decades in the field of bioremediation, namely the use of microorganisms and their enzymes to remove pollutants in the Pseudomonas putida can destroy various types of organic compounds, such as aromatic compounds, aliphatic compounds, and sugar.
It is suitable for bioremediation of contaminated soil and groundwater due to its metabolic flexibility and durability and ease handling its genetics.
putida is useful in the bioremediation of polluted sites due to its ability to destroy complex aromatic compounds such as xylene, toluene, and aromatic nitro putida lives in many places, such as polluted land, air and environment.
Since the 60s, the ability of bacteria to destroy xenobiotics has been known.
Since then, research on this bacterium has developed rapidly.
The rod-shaped gram-negative bacterium Pseudomonas putida has great potential for the biotechnology industry.
It is suitable for various applications due to its beneficial metabolism, rapid growth, and resistance to harsh 1 P.
putida is attracting attention due to its potential in industrial biotechnology in addition to its environmental applications.
putida can produce certain chemicals or perform certain functions, making them attractive candidates for bioprocesses and biocatalysis.
Additionally, it can produce and discharge valuable chemicals such as biofuels, biopolymers and platform chemicals.
1193 | The Role of Pseudomonas Putida in Bioremediation: an Overview (Andi Tirta Angraen.
Jurnal Ilmu Sosial dan Pendidikan (JISIP) e-ISSN : 2656-6753, p-ISSN: 2598-9944 This article will discuss various aspects of Pseudomonas putida in bioremediation.
will also discuss potential applications of P.
putida in the environmental bioremediation and biotechnology industry.
By understanding the unique properties of P.
putida and its potential applications, we can harness its power to provide sustainable solutions to environmental pollution and contribute to the development of a more sustainable and biobased economy.
MATERIAL
Pseudomonas putida is a bacterium that is often the object of research in microbiology because of its ability to adapt to the environment and form biofilms as a protective mechanism against stressful conditions.
14 Cell cells P.
putida planktonic to the inner surface of the silicon tube to form a biofilm.
4 Adaptation mechanisms P.
putida to abiotic These mechanisms include.
Membrane barrier adaptation, changes in the composition and fluidity of cell membranes to maintain cellular integrity under stress.
Phosphate absorption, taking up and using phosphate to maintain cellular function.
Maintenance of intracellular pH and redox status, controlling cytoplasmic pH and redox status to protect cellular proteins and macromolecules.
Metabolic translational control, controlling gene expression and translation.
Pseudomonas putida A highly flexible bacterium, it is an important subject in microbiological research due to its biofilm formation and ability to adapt to a wide range of environmental conditions.
Its metabolic flexibility allows it to degrade a wide range of organic compounds, making it ideal for bioremediation of polluted soil and water.
The formation of bacterial biofilms increases their survival under stressful conditions, thereby creating a powerful bioremediation system.
This species of bacteria is very common in the environment, and can be found in the air, soil, and rhizosphere of plants.
They play an important role in ecology, with some strains improving plant growth and soil health, while others are involved in the biodegradation of xenobiotic compounds.
However, current taxonomic classifications, which are based on phenotypic characteristics such as metabolic assays, fatty acid composition, and protein profiles, are considered outdated and inadequate.
5 This organism is well suited for the biotechnological production of various valuable compounds due to its genetic advantages, versatile metabolism, and tolerance to xenobiotics, plus its GRAS status.
Full potential P.
putida as a cell factory for the production of sustainable and environmentally friendly natural products will be discovered by continuous research.
Pseudomonas putida has very broad potential to function as a biotechnology platform to produce various very valuable compounds, but this potential is still not widely used.
Further research is needed to fully unlock the potential of this versatile and powerful bacterium, as well as to develop sustainable and environmentally friendly bioprocesses for making a wide range of products using P.
putida as a microbial cell factory.
With its unique genetic and metabolic characteristics.
putida has the potential to revolutionize the biotechnology industry and support a sustainable, bio-based economy.
Acid tolerance P.
putida through genetic engineering by inserting the GAD system and the IrrE regulator.
The engineered strain showed enhanced viability and biodegradation activity under acidic conditions, paving the way for its promising use in acidic waste the potential of synthetic biology to develop microorganisms to better address environmental problems.
3 stress tolerance in bacteria and can be used to increase biotechnology adoption P.
putida, such as bioremediation and bioproduct production.
Identifying specific genes and pathways involved in the stress response can be used to engineer strains P.
putida which is more resistant to harsh environmental conditions.
6 More research is needed to fully see the potential P.
putida and developing scalable 1194 | The Role of Pseudomonas Putida in Bioremediation: an Overview (Andi Tirta Angraen.
Jurnal Ilmu Sosial dan Pendidikan (JISIP) e-ISSN : 2656-6753, p-ISSN: 2598-9944 biotechnology solutions for various industries.
Strains P.
putida Genetically engineered ones with better tolerance to environmental stress could enable the use of these versatile bacteria in a variety of biotechnological applications.
Industrial Applications P.
putida has been used in various industrial applications, such as bioremediation, involving pollutants and xenobiotics.
Chemical production, manufacture of bulk and specialty chemicals, natural products .
hamnolipids, terpenoids, polyketides, non-ribosomal peptide.
, and biopolymers.
Biocatalyst, catalyzes biochemical reactions using enzymes.
1 Since conventional physico-chemical methods are often inefficient and expensive in dealing with organic pollutants that persist in soil and air, biotechnology is a cost-effective and innovative approach to protecting the environment, especially in the management of hazardous waste from industry.
In industrial use.
Pseudomonas putida offers great opportunities to develop sustainable and environmentally friendly bioprocesses.
We can harness the power of these versatile and powerful bacteria to address many of the most important environmental problems, such as bioremediation, waste treatment, and the production of valuable compounds using renewable carbon sources.
To fully unlock potential P.
putida and developing scalable and useful biotechnology applications for various industries.
METHOD
This research uses a qualitative descriptive approach to collect data through literature Literature is selected based on criteria such as relevance to the topic, novelty of the information, and credibility of the source.
After that, the literature is explained Thorough reading is necessary to find similarities or differences in opinion.
Literature analysis will be used to support the arguments in the article by supporting the ideas presented to develop the necessary discussions, findings and conclusions.
Relevant reference lists were also searched manually.
In this article, every publication relevant to the above basic data is included and thoroughly discussed.
RESULTS AND DISCUSSION
Pseudomonas putida are bacteria that play an important role in the field of environmental biotechnology, especially in bioremediation efforts.
Its ability to degrade various hazardous substances, such as heavy metals, hydrocarbons and industrial waste, makes it an efficient biological agent for reducing environmental pollution.
3 Bacterial genomes such as Pseudomonas putida improving bioremediation by simplifying it by removing non-essential genes and optimizing metabolic pathways involved in pollutant 18 By modifying their metabolic pathways, scientists have enhanced the ability of these bacteria to produce high-value chemicals such as biofuels and bioplastics from renewable resources.
This innovation opens up opportunities for the production of environmentally friendly chemicals on an industrial scale, making Pseudomonas putida as a major player in sustainable industrial biotechnology in the future.
According to research that looks at how P.
putida interacts with plants, these bacteria can improve plant growth by increasing nutrient supply and protecting plants from Thus.
Pseudomonas putida helps bioremediation and ensures the extinction of agriculture, being one of the microorganisms that is very important for maintaining ecosystem balance and supporting nutrient availability.
Bioremediation is a natural process that utilizes microorganisms, such as Pseudomonas putida, to decompose and remove pollutants, including petroleum hydrocarbons, from polluted environments.
15 The bioremediation process involves several stages, namely identification of biosurfactant-producing microorganisms, biosurfactant production, 1195 | The Role of Pseudomonas Putida in Bioremediation: an Overview (Andi Tirta Angraen.
Jurnal Ilmu Sosial dan Pendidikan (JISIP) e-ISSN : 2656-6753, p-ISSN: 2598-9944 application to contaminated soil, oil degradation by microorganisms, as well as monitoring and evaluating the results of the process.
Microorganisms that produce biosurfactants are important because they can increase the solubility and bioavailability of hydrocarbons, which makes it easier for microorganisms to access and destroy pollutants.
Monitoring and evaluating results is critical to achieving success in the bioremediation process, ensuring that pollutants have been effectively destroyed and that the environment has returned to a healthier condition.
Bioremediation, which uses microorganisms to clean pollutants, offers an environmentally friendly and inexpensive alternative.
Ability Pseudomonas putida to deal with oil hydrocarbon pollutants.
By using BHM media with crude oil as the sole carbon source, the ability to oxidize crude oil under ideal conditions shows tremendous potential.
With the ability to decompose complex hydrocarbon compounds.
Pseudomonas putida plays an important role in the oil spill bioremediation process.
Through their enzymatic activity, these bacteria can convert parts of petroleum into simpler and non-toxic compounds, which helps reduce environmental pollution levels.
17 Study of how Pseudomonas putida interacting with other microorganisms in the ecosystem can help develop more efficient and sustainable bioremediation methods.
Thus.
Pseudomonas putida not only does it rid the environment of pollutants, but it can also be an important part of a long-term solution to increasingly complex contamination problems.
Biodegradation of crude oil is influenced by temperature, microbial composition, and Due to the different oil fractions, a diverse microbial community is essential.
putida also grows.
I am milking the vines .
lack bean.
in oil-contaminated soil.
These results show that P.
putida can function as a bioremediation agent that has the potential to clean and restore the fertility of soil contaminated by crude oil.
10 A study of the interaction between P.
putida And I am milking the vines can generate new ideas about better bioremediation methods.
It could also show how a combination of microbes and plants can be used to remediate contaminated land.
With this method, pollution can not only be overcome, but soil fertility can also be reduced, thereby helping the sustainability of agriculture in the future.
Pseudomonas putida consists of various strains with special characteristics that enable their use in various fields, such as biotechnology, bioremediation and agriculture.
Apart from that, the KT2440 strain is known as a safe mutant and is not pathogenic, so it is often used as a host for cloning soil bacterial genes.
12 The T6SS serves as a possible molecular tool P.
putida sends toxic effector proteins into the cells of competing bacteria and plant pathogens, so that they can inhibit the growth or even eliminate the target 13 P.
putida 1274 can break down PNP well, especially under ideal temperature and pH conditions, with hydroquinone as the main product.
The success of this relegation shows that P.
putida 1274 has the ability as a bioremediation agent to clean environments contaminated with PNP.
8 Therefore.
Pseudomonas putida improving plant health and agricultural productivity, in addition to helping bioremediation and Potential of microorganisms in the pollutant bioremediation process.
While cow dung microflora has the ability to degrade benzene up to 100% at low concentrations.
Pseudomonas putida MHF 7109 was identified as an effective candidate for degrading In different research, a two-phase partition bioreactor was used to break down Pseudomonas putida IFO 14671 functions as an effective phenol degrader.
The second research shows that bioremediation can be used as an environmentally friendly way to deal with phenol and benzene pollution.
2 The ability of Pseudomonas to support bioremediation is not only limited to the degradation of hazardous compounds, but also contributes to the utilization of organic waste as a renewable resource.
16 Apart from 1196 | The Role of Pseudomonas Putida in Bioremediation: an Overview (Andi Tirta Angraen.
Jurnal Ilmu Sosial dan Pendidikan (JISIP) e-ISSN : 2656-6753, p-ISSN: 2598-9944 functioning as a bioremediation agent.
Pseudomonas putida also plays an important role in the transition towards a more environmentally friendly circular economy.
Further research into genetic variations and bacterial metabolic mechanisms could enable the creation of strains that are better at waste utilization and pollutant degradation.
CONCLUSION
The research results show that Pseudomonas putida is very important for environmental biotechnology, especially for reducing environmental pollution.
This is due to its ability to destroy hydrocarbons, heavy metals and industrial waste.
Additionally, by reducing the depletion of nonessential genes and optimizing the metabolic pathways involved in pollutant expression, the genome of these bacteria can be altered.
With this innovation, environmentally friendly chemicals can be produced on an industrial scale, and Pseudomonas putida will be a major player in the sustainable biotechnology industry of the future.
Bioremediation and biotechnology assistance.
Pseudomonas putida also improves plant health and agricultural productivity.
With its unique genetic and metabolic characteristics, putida has the potential to revolutionize the biotechnology industry and support a biobased and sustainable economy.
However, the potential of this versatile and powerful bacterium is still not widely used, and further research is needed to fully explore the potential of this versatile and powerful bacterium and to develop sustainable and environmentally friendly bioprocesses for making a wide range of products using P.
Ability to overcome petroleum hydrocarbon pollutants and benzene shows that these bacteria can be used as good bioremediation agents to reduce pollution levels in the Besides that.
putida plays an important role in utilizing organic waste as a renewable resource and helping build a circular economy that is more environmentally BIBLIOGRAPHY