Evaluation of Erythrocyte Sedimentation Rate (ESR) of Motorcycle Workshop Worker Exposed to Benzene Moch. Sahri1. Maharani Pertiwi Koentjoro2. Shamsul Bahari Shamsudin3 Department of Occupational Health and Safety. Faculty of Health. Nahdlatul Ulama University of Surabaya. Indonesia Department of Medical Laboratory Technology. Faculty of Health. Nahdlatul Ulama University of Surabaya. Indonesia Department of Community & Family Medicine. Faculty of Medicine & Health Sciences. Universiti Malaysia Sabah. Malaysia Correspondence: Maharani Pertiwi Koentjoro. Jl. Jemursari No. Surabaya. East Java. Indonesia Zip Code : 60237 Email: maharani@unusa. Received: September 9, 2019 Revised: October 7, 2019 Accepted: October 12, 2019 Abstract Occupational exposure to benzene from fuel oil in workshop motorcycle workshop causes several health effects, depending upon the level and duration of exposure. Analysis of blood is important to access the status of workerAo health. The study aimed to assess the gather basic information required for protecting workersAo health and improve working conditions in the works sites by investigating the ESR levels. A comparative cross-sectional study conducted in Surabaya City. East Java-Indonesia, which involved 100 The occupational data collected using a structured questionnaire while blood analysis parameters measured with an automated hematology analyzer. The result showed that leukocytes, erythrocytes, hemoglobin (HGB), hematocrit values (HCT), platelet. MCV. RDW, eosinophil, basophils, neutrophil rods, neutrophil segment, lymphocyte, monocytes were in normal value. Whereas. MCH. MCHC. ESR has shown in abnormal value as average 24. 5 pg, 29. g/dL and 8. 21 mm/h, respectively. Those value, especially in ESR value indicated the increasing concentration plasma This is may cause by inflammation in the workersAo To prevent the hazardous effect of benzene exposure, occupational health should be implemented for workers in order to protect them from exposure to benzene. Keywords Motorcycle workshop workers. ESR. Surabaya city, sites process various chemical products based INTRODUCTION The level of benzene exposure in the on highly volatile and flammable liquid workshop during hydrocarbons . , . , namely Premium, operations may occur. Mechanical workshop Pertamax. Solar which are the most widely workshop motorcycle Moch. Sahri, et al. used types of fuel oil. It presents many dangers associated with toxic chemical protecting workersAo health and improve materials, including carcinogens such as working conditions during works sites by However, recent reports have investigating the hematological parameters, indicated a link between occupational especially in the ESR levels. diseases such as cancer and benzene exposure in workshop site . Workers in sites MATERIALS AND METHODS exposure to benzene are more risk in Study design occupational health . , . A comparative cross-sectional study of Erythrocyte Sedimentation Rate (ESR) is workers conducted at a workshop motorcycle a blood test that measures a Aused rateAy. This The study subject involved 100 rate is depends on various factors, namely hemoglobin concentration, the ratio of workers exposed to fuel oil. All worker plasma proteins, serum lipid concentration, require hematological parameters evaluation, and plasma pH. It has been a widely used especially in ESR levels. All workers were marker for inflammation and indicates asked to give informed consent. In the occupational health of fuel Data collection oil. ESR determination is an important part of Demographic and occupational data were critical and emergent diagnoses Duc as acute collected using a structured questionnaire, and chronic lymphocytic leukemia. and The subjects Ade interviewed by the HodgkinAos lymphoma, multiple myeloma. Before the blood collection, resting blood pressure was measured by using dysfunction, malignancy, temporal arteritis. Blood samples from renal disease, collagen vascular disease . worker respondents were taken at the end of . Furthermore, the role of ESR in the the work shift. 4 mL of blood samples were clinical management of various disease . collected using venipuncture techniques into Routine a Vacutainer tube containing potassium motorcycle workshop include such as EDTA replacing spark plugs or changing the oil. The tubes were then placed in a repair engines and transmissions, removing dents from fenders. Thus, evaluation of the undisturbedly inin cold box. All samples effect of benzene exposure is one of occupational health requirement at workshop hematology analyzer Horiba Type ABX motorcycle workshop . This study aims to Micros 60 (KX-21N. Sysmex. Kobe. Japa. placed/stored Ina J Med Lab Sci Tech 2019. : 52-57 Moch. Sahri, et al. RESULTS Data processing and analysis General characteristics All the data were cleaned, double entered Personal characteristics of the workshop into Microsoft Excel spreadsheets and motorcycle workers are presented in Table 1 analyzed using Stata software version 13 They were comparable with regard to age and (StataCorp. College Station. TX. USA). 91% of workers were males. The average Ethical considerations age of the workers was 28 years. Protective The proposal in this study was approved by the Surabaya occupational safety and measures were poorly followed during work in workshop. health technical implementation unit. respondents in this study gave written Personal Charateristics of Workshop Motorcycle Workers Table 1. agreements to participate in this study. All respondents were given an explanation of the purpose and results of this study. Laboratory test results were given to respondents in this Age Group Range Age-group . Percentage (%) Female Male Sex Table 2. Major Activities of The Workshop Motorcycle Workers Job Task Dismantle engines and repair or replace defective parts, such as Mechanical magnetos, carburetors, and generators. Remove cylinder heads, grind valves, and scrape off carbon, and replace defective valves, pistons, cylinders and rings, using hand tools and power Hammer out dents and bends in frames, weld tears and breaks. reassemble frames and reinstall engines. Repair or replace other parts, such as headlights, horns, handlebar controls, gasoline and oil tanks, starters, and mufflers. Repair and transmissions, brakes, and drive chains, according to specifications. Ina J Med Lab Sci Tech 2019. : 52-57 Moch. Sahri, et al. Fig 1. Partial view of working practice in of the workshop motorcycle. Workers are engaged in routine activities without proper personal protective equipment . ask and glove. Based on this study, the possible routes of chemical entry into the body trough inhalation. has shown in Figure 1 that the workers did not wear a mask during workpractice Table 3. Blood Analysis and Hematological Parameters of Workshop Motorcycle Workers Parameters Average value Normal (%) Abnormal (%) 53 x10 /mm Leukocytes 07 x 106/mm3 Erythrocytes 51 g/dL Hemoglobin (HGB) Hematocrit values (HCT) Platelet 36 AAmA MCV 5 pg MCH 18 g/dL MCHC RDW Eosinofil Basophils Natrofil rod Neutrophil Segment Lymphocyte Monocytes 21 mm/h ESR MCV (Mean Corpuscular Volum. MCH (Mean Corpuscular Hemoglobi. MCHC (Mean Concentratio. RDW (RBC Distribution Widt. ESR (Erythrocyte Sedimentation Rat. DISCUSSION