Anti-thrombocytopenic Activity of Ethnomedicinal Plants from Indonesia (Susilawati Y, et al.
Indones Biomed J.
: 279-86
DOI: 10.
18585/inabj.
RESEARCH ARTICLE
Anti-thrombocytopenic Activities of 15 Selected Indonesian Medicinal Plants:
Psidium guajava Leaves.
Phyllanthus niruri Aerial Parts, and Andrographis paniculata Leaves as Potential Sources in Enhancing Platelet Levels in Rats Yasmiwar Susilawati1,2E.
Ami Tjitraresmi1.
Ferry Ferdiansyah Sofian1.
Eli Halimah3.
Humaira Praswatika Dewi1.
Aria Aristokrat4 Department of Biological Pharmacy.
Faculty of Pharmacy.
Universitas Padjadjaran.
Jl.
Raya Bandung Sumedang Km.
Jatinangor 45363.
Indonesia Herbal Study Center.
Faculty of Pharmacy.
Universitas Padjadjaran.
Jl.
Raya Bandung.
Sumedang Km.
Jatinangor 45363.
Indonesia Department of Pharmacology and Clinical Pharmacy.
Faculty of Pharmacy.
Universitas Padjadjaran.
Jl.
Raya Bandung Sumedang Km.
Jatinangor 45363.
Indonesia Apotechary Study Program.
Faculty of Pharmacy.
Universitas Padjadjaran.
Jl.
Raya Bandung Sumedang Km.
Jatinangor 45363.
Indonesia *Corresponding author.
Email: yasmiwar@unpad.
Received date: Feb 18, 2025.
Revised date: May 14, 2025.
Accepted date: May 26, 2025 Abstract ACKGROUND: Thrombocytopenia is a medical condition characterized by a low platelet count in the blood, which can cause excessive bleeding and serious complications.
Current conventional treatments for thrombocytopenia often present significant side effects and limitations, necessitating the exploration of alternative therapeutic options.
Therefore, this study was conducted to evaluate the potential of Indonesian ethnomedicinal plants as alternative therapies.
METHODS: The research was conducted by ethanol maceration of 15 medicinal plants selected based on their use in traditional Indonesian medicine to treat thrombocytopenia.
Male white rats, aged three month and weighing between 150 and 250 g, were divided into four groups, which each group consists of five rats: the negative control group, group receiving 250 mg/kg BW extract, group receiving 500 mg/kg BW extract, and the normal control group.
The effects of the test preparations on platelet count, erythrocyte count, and hematocrit were observed after 7 days of treatment.
RESULTS: Among of 15 medicinal plants extract tested at a dose of 500 mg/kg BW.
Psidium guajava L.
leaves extract showed to increase blood platelets counts in thrombocytopenic mice by 60.
82% .
<0.
followed by Phyllanthus niruri aerial parts extract at .
p<0.
, and Andrographis paniculata (Burm.
) Wall.
ex Nees leaves extract .
p<0.
CONCLUSION: The extracts of P.
guajava leaves, aerial parts of P.
niruri, and A.
paniculata leaves exhibited significant potential in enhancing platelet levels.
These findings suggest that some Indonesian medicinal plants can be used as alternative therapy for thrombocytopenia.
KEYWORDS: dengue hemorrhagic fever.
Phyllanthus niruri.
Psidium guajava.
Andrographis paniculata, thrombocytopenia Indones Biomed J.
: 279-86
Introduction Dengue Hemorrhagic Fever (DHF) has become an endemic disease that occurs almost all over the world.
The most infected areas are tropical regions, such as Southeast Asia and the Western Pacific.
The disease is caused by the rapid transmission of the Aedes aegypti mosquito, and the cases in Indonesia have ranked second among endemic countries.
According to data from the Ministry of Health of the Republic of Indonesia, dengue cases in March 2024 had reached 53,131 and the death rate was 404 people.
The prevalence of these cases tripled from 2023 with 17,434 cases and 118 deaths.
Transmission of the infection starts Copyright A 2025 The Prodia Education and Research Institute.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.
0 International (CC-BY-NC) License.
The Indonesian Biomedical Journal.
Vol.
No.
June 2025, p.
Print ISSN: 2085-3297.
Online ISSN: 2355-9179 with a mosquito bite, which then the virus enters the blood vessels and causes thrombocytopenia.
Interpretation of the severity of DHF disease experienced can be seen from the Thrombocytopenia, a hematological condition characterized by a platelet count lower than the normal physiological range, is a dangerous condition which leads to the formation of thrombosis.
It can trigger various problems in the cardiovascular system, such as acute coronary Platelets, also known as thrombocytes, play a crucial role in the hemostatic process by facilitating blood clot formation.
A reduced platelet count compromises the bodyAos ability to effectively form clots, leading to challenges in halting bleeding and increasing the risk of hemorrhagic .
Treatment of thrombocytopenia is commonly referred to as anti-thrombocytopenic, usually uses glucocorticoids, immunoglobulins, thrombopoietin receptor agonists (TPORA.
, splenectomy, rituximab, and immunosuppressants.
These treatments have proven to have good effectiveness in increasing platelet production and inhibiting autoantibody However, there are some adverse side effects, such as TPO-RAs that can increase the risk of thrombosis.
This is due to its mechanism of increasing platelet count and stimulating platelet production.
In addition, shortterm side effects of glucocorticoid use can cause insomnia, dyspepsia, and hyperglycemia.
Vaccines can be used to prevent DHF.
However, vaccines have several constraints such as age, side effects, long-term effects, and a history of .
Therefore, alternative therapies using natural products are needed.
Indonesian people often use natural ingredients, specifically plants that are known from generation to generation to increase blood platelet levels.
The natural ingredients that are commonly used by Indonesians for this purpose include papaya leaves (Carica papaya L.
) and guava fruit (Psidium guajava L.
In addition, plants such as aerial parts of alang-alang (Imperata cylindrica (L.
) Beauv.
), sambiloto leaves (Andrographis paniculata (Burm.
) Wall.
ex Nee.
, aerial parts of pegagan (Centella asiatica (L.
) Urba.
, dewa leaves (Gynura divaricata (L.
) D.
C), sweet potato leaves (Ipomoea batatas L.
Poir.
temu ireng rhizomes (Curcuma aeruginosa Roxb.
kencur rhizomes (Kaempferia galanga L.
), tapak liman leaves (Elephantopus scaber L.
), red betel leaves (Piper crocatum Ruiz & Pa.
, green beans (Vigna radiata (L.
Wilcze.
, rosella (Hibiscus sabdariffa L.
), and aerial parts of meniran (Phyllantus niruri L.
) are also often used by the community to raise platelets.
Results of a survey of herbal medicine use in the community in West Java.
Indonesia also showed that these plants are often used especially in the treatment of DHF, especially in increasing platelet counts because they contain compounds such as flavonoids especially quercetin.
However, information on the efficacy of these plants is still unclear and there is no scientific evidence so that their use is without clear rules and .
Therefore, further research is needed as scientific evidence regarding the effectiveness of therapy with the use of these plants.
Alternative anti-thrombocytopenia derived from plants is needed and expected to have low side effects and low toxicity to the body.
The high number of dengue cases in Indonesia and other tropical areas and the lack of drugs that effectively increase platelet levels make research on the anti-thrombocytopenic activity of plants essential.
In addition, research on the effects of anti-thrombocytopenia has not been widely studied.
It is hoped that these plants can be developed as an alternative therapy in increasing the number of platelets in the blood.
So, this research aims to prove the effects of ethnomedicine plants commonly used to treat thrombocytopenia using test animals, specifically white rats.
Methods Sampling and Determinations The plants used in this study were selected based on the results of literature studies regarding plants that were traditionally used for the treatment of DHF cases.
The plants were collected from The Manoko Experiment Garden (Lembang.
Indonesi.
The plant materials was produced by drying the cleaned parts of the plant in indirect sunlight.
The determination was carried out at the Plant Taxonomy Laboratory.
Department of Biology.
Faculty of Mathematics and Natural Sciences.
Universitas Padjadjaran.
Bandung (No.
476/HB/01/2.
Determination results were shown in Table 1.
Extraction Process Extraction was performed to each medicinal plant using a maceration method with 70% ethanol solvent for 3y24 hours with solvent replacement every 24 hours.
The extract was collected in an Erlenmeyer flask and then concentrated using a rotary evaporator at a temperature higher than 40AC.
To determine the amount of condensed extract, the yield calculation was carried out.
The results of the extract yield calculation from the 15 plants were also listed in Table 1.
DOI: 10.
18585/inabj.
Anti-thrombocytopenic Activity of Ethnomedicinal Plants from Indonesia (Susilawati Y, et al.
Indones Biomed J.
: 279-86
Table 1.
Taxonomy and yield result of 15 medicinal plants used.
Spesies Imperata cylindrica (L.
) Beauv.
Family Local Name Parts Used Quantity .
Yield (%) Poaceae Alang-alang Aerial parts Andrographis paniculata (Burm.
) Wall.
ex Nees Psidium guajava L.
Centella asiatica (L.
) Urban Gynura pseudochina (L.
) D.
Ipomoea batatas (L.
) Poir.
Acanthaceae Myrtaceae Apiaceae Asteraceae Convolvulaceae Sambiloto Jambu Biji Herba Pegagan Dewa Ubi Jalar Leaves Fruits Aerial parts Leaves Leaves Curcuma aeruginosa Roxb.
Zingiberaceae Temu Ireng Rhizome Kaempferia galanga L.
Elephantopus scaber L.
Zingiberaceae Asteraceae Kencur Tapak Liman Rhizome Leaves Piper crocatum Ruiz & Pav.
Vigna radiata (L.
) R.
Wilczek Piperaceae Fabaceae Sirih Merah Kacang Hijau Leaves Seed Hibiscus sabdariffa L.
Psidium guajava L.
Malvaceae Myrtaceae Rosella Jambu Biji Merah Flower Leaves Phyllanthus niruri L.
Carica papaya L.
Euphorbiaceae Caricaceae Herba Meniran Pepaya Aerial parts Leaves Animal Model The animals used were male white rats (Rattus norvegicu.
Wistar strain aged 3 months weighing 150 to 250 grams.
The test animals were obtained from D'Wistar.
Bandung (No.
RL/D'WISTAR/20.
Rat cages were lined with wood shavings because they were easy to replace, can absorb liquids, have no pungent odor, and are free of chemicals.
Each cage was equipped with food and drink containers.
Before use, the test animals were acclimatized for 7 days.
The body weight of the test animals was weighed, and their behavior was observed periodically.
Animals are declared fit and can be used if they do not lose more than 10% of their .
The protocol experiment had been approved by the Research Ethics Commission of Universitas Padjadjaran (No.
620/UN6.
KEP/EC/2.
Anti-thrombocytopenic Activity Test All 15 test extracts were tested against Wistar male white rats for to analyze its anto-thrombocytopenic activity.
Rats were divided into 4 test groups, each group consisted of 5 rats, including: the negative control group, test group 1 which received 250 mg/kg BW of each extract, test group 2 which received 500 mg/kg BW of each extract, and the normal control group.
Animals in the negative control group were only administered with 150 IU Heparin (NaHeparin.
Inviclot.
Fahrenheit.
Jakarta.
Indonesi.
solution inducer intraperitoneal.
Animals in test group 1 and 2 were induced with the same dosage of heparin, then given the extracts orally at a dose of 250 mg/kg BW and 500 mg/kg BW, respectively.
Animals in the normal control group did not receive any treatment and were observed at their normal For 7 days, the rats in test group 1 and 2 were given the test extract.
The blood of the rats was taken on the 7th day after treatment, so that the number of platelet, erythrocyte, and hematocrit levels can be calculated (Figure .
Rat blood was collected by putting the rats into an isolation cage, after which the rat's tail was cleaned with 70% alcohol using a cotton swab.
The rat's tail was irradiated with a lamp with a distance of 30 cm for 15 minutes to dilate the blood vessels.
The rat tail was cut approximately 0.
5 cm from the tip and the blood was collected in an anticoagulantcoated blood tube.
In calculating the number of platelet, erythrocyte, and hematocrit counts, the Sysmex XE-5000 hematology analyzer (Sysmex America.
Lincolnshire.
IL.
USA) was used.
Statistical Analysis Statistical analyses were performed using Statistical Package for Social Science (SPSS) version 29 (IBM Corporation.
Armonk.
NY.
USA).
The normality of the data was tested with the Shapiro-Wilk test.
The data analysis was done with the Independent T-Test for the parametric tests.
The confidence interval in this study was 95% so that if the p<0.
05 then it could be stated meaningful in statistics.
Results Platelet.
Erythrocyte and Hematocrit Counts in Negative and Normal Control The number of platelet, erythrocyte and hematocrit in each blood was observed.
The data for each test extract was then compared with the negative control to get the percentage The Indonesian Biomedical Journal.
Vol.
No.
June 2025, p.
Print ISSN: 2085-3297.
Online ISSN: 2355-9179 Figure 1.
Study scheme and timeline of the in vivo antithrombocytopenic activity test.
value of the increase in platelet, erythrocyte and hematocrit, so that it can make it easier to see which test extract has the best anti-thrombocytopenic activity.
The average value of platelet, erythrocyte and hematocrit counts in normal control group before treatment 75A0.
16y105, 10.
58A0.
27y105, and 51.
25A0.
Whereas the platelet, erythrocyte, and hematocrit counts on the 7th day of the negative control and normal control could be seen in Table 2.
Platelet.
Erythrocyte and Hematocrit Counts Increased After the Administration of Extracts The average increase in platelet, erythrocyte and hematocrit counts after treatment of the 15 extracts at dose of 250 mg/kg BW and 500 mg/kg BW were presented in Table It was found that almost all test extracts gave antithrombocytopenic activity.
This was shown by the increase in the value of platelet counts in experimental animals after administration of plant extracts at the dose of 250 mg/kg BW and 500 mg/kg BW 62% and 6.
82%, respectively.
leaves, aerial parts of P.
niruri, and A.
paniculata leaves provide the best platelet-raising .
nti-thrombocytopeni.
activity at a dose of 500 mg/kg BW.
These results were supported by statistical tests showing a significant difference .
<0.
in the increase in platelet count after treatment with extracts of P.
niruri, and A.
The largest average increase in the number of erythrocytes was given by C.
aeruginosa extract at a dose of 500 mg/kg BW, and 250 mg/kg BW and P.
guajava leaves at a dose of 500 mg/kg BW.
This shows that besides having the highest anti-thrombocytopenic activity.
guajava leaves can also significantly increase the number of erythrocytes or red blood cells.
Meanwhile the average percentage of blood hematocrit rats on the 7th day showed the greatest increased after given 500 mg/kg BW G.
From the observations of the increase in platelet, erythrocyte and hematocrit, the potential extracts for making natural herbal medicine preparations for thrombocytopenia which also increase erythrocyte levels and do not trigger plasma leakage were the aerial parts of P.
niruri at a dose of 500 mg/kg BW and P.
guajava leaves both at doses of 250 and 500 mg/kg BW).
Discussion From the 15 extracts, there are 3 extracts that shown the greatest effects in increasing blood platelets, namely P.
guajava leaves.
niruri aerial parts, and A.
paniculata leaves.
The increase in blood platelet levels in the three plants was significantly different based on statistical .
<0.
Results of the experiment showed that the administration of 250 and 500 mg/kg BW of the test preparation increases the platelet, erythrocyte, and hematocrit counts in the rats.
The three parameters were measured because they are interconnected with each other.
Low platelets can trigger plasma leakage which is indicated by an increase in hematocrit.
While hematocrit will affect erythrocytes.
In this study, heparin was used to induced Heparin induction resulted in a 53.
decrease in platelet levels in the test animals (Table .
Anti-thrombocytopenic Activity of Ethnomedicinal Plants from Indonesia (Susilawati Y, et al.
Indones Biomed J.
: 279-86
DOI: 10.
18585/inabj.
Table 2.
Average results of the platelet, erythrocyte and hematocrit counts in rat blood on the 7th day observation .
Group Platelet Count Negative Normal Erythrocyte Count 75A0.
43 x 10 69A0.
14 x 10 5A0.
27A0.
59A0.
55 x 10 29A0.
33 x 10 Additionally, there was a decrease of 28.
26% in erythrocyte levels and 11.
21% in hematocrit levels.
This is in accordance with another previous study, where after heparin exposure, platelet numbers decline rapidly, sometimes by 50% or more from baseline in 90% respondent.
From Table 3, the administration of the extract at the test dose did not cause an increase in hematocrit above 20%, so that the administration of the test extract will not trigger plasma permeation in dengue fever subjects.
guajava from the Myrtaceae family contain quercetin, avicularin, apigenin, guaijaverin, kaempferol, hyperin, myricetin, gallic acid, catechin, epicatechin, chlorogenic acid, epigallocatechin gallate, and caffeic acid.
guajava leaves extract have several pharmacological activities such as anticancer .
, antioxidant, antidiabetic .
, antimicrobial .
, and hepatoprotective .
Aqueous extracts of P.
guajava leaves were found to significantly increase platelet count after cyclophosphamide-induced The leaf extract has benefits such as reducing the severity of dengue bleeding.
From the results of the phytochemical screening, polyphenols, tannins, saponins, flavonoids, monoterpenoids, sesquiterpenoids, triterpenoids, and quinones were found in the extract.
Hematocrit Count From the experimental results, it was found that ethanol extract of P.
guajava leaves increases the number of platelet, erythrocyte, and hematocrit counts.
This is also proportional to the increase in dose, the higher the dose given, the greater the increase in the number of parameters The flavonoid content of quercetin can increase platelet count because it is assisted by amino acids which have a function as a building substance that can affect thrombopoietin, which is a megakaryocyte stimulating .
This finding is also supported by previous study that has proven the megakaryocyte increasing activity of P.
guajava leaves extract in white Wistar rats.
Megakaryocytes are the precursor of platelets.
Therefore, the increase of megakaryocytes could help in increasing the platelet counts.
Not only the leaves.
guajava fruit extract is also shown to have anti-thrombocytopenic activity through the enhancement of stem cell factor niruri is a plant that belongs to the Euphorbiaceae This plant has several activities such as antiviral activities because it can inhibit DNA polymerase, can increase the body's immune system, antioxidant, antibacterial, antifungal, and can treat dengue fever.
This Table 3.
Average results of the increase in platelet, erythrocyte and hematocrit counts in rat after receiving plant extracts .
Increase in Platelet Count (%) Increase in Erythrocyte Count (%) Increase in Hematocrit Count (%) 250 mg/kg BW 500 mg/kg BW 250 mg/kg BW 500 mg/kg BW 250 mg/kg BW 500 mg/kg BW Imperata cylindrica (L.
) Beauv.
Andrographis paniculata (Burm.
Wall.
ex Nees 55A1.
39A0.
68A3.
17A0.
34A0.
52A1.
05A0.
44A2.
37A0.
96A1.
14A0.
15A1.
Psidium guajava L.
(Frui.
Centella asiatica (L.
) Urban 35A3.
19A0.
27A6.
00A1.
57A1.
11A1.
12A1.
05A0.
85A0.
54A0.
22A1.
19A0.
Gynura pseudochina (L.
) D.
Ipomoea batatas (L.
) Poir.
Curcuma aeruginosa Roxb.
Kaempferia galanga L.
Elephantopus scaber L.
Piper crocatum Ruiz & Pav.
12A0.
74A0.
84A1.
28A0.
51A0.
33A0.
03A0.
13A1.
27A6.
47A0.
37A0.
66A1.
44A3.
62A1.
83A1.
64A0.
21A1.
52A0.
44A4.
95A0.
98A1.
48A1.
99A0.
99A1.
94A1.
68A1.
28A0.
12A0.
13A0.
34A1.
97A0.
08A1.
2A0.
28A0.
55A0.
72A1.
Vigna radiata (L.
) R.
Wilczek 21A4.
47A3.
61A1.
56A1.
32A0.
19A1.
Hibiscus sabdariffa L.
Psidium guajava L.
(Leave.
81A7.
62A4.
28A9.
82A8.
62A0.
1A1.
45A1.
41A1.
12A1.
65A1.
77A1.
13A2.
Phyllanthus niruri L.
Carica papaya L.
27A1.
26A3.
22A1.
62A3.
1A0.
28A3.
43A1.
18A1.
03A1.
09A0.
12A0.
39A0.
Plant The Indonesian Biomedical Journal.
Vol.
No.
June 2025, p.
Print ISSN: 2085-3297.
Online ISSN: 2355-9179 is because P.
niruri contains several compounds, including tannins, quinones, triterpenoids, flavonoids, essential oils, coumarins, and polyphenols.
Bioactive compounds from niruri are niruriside which has antiviral activity .
and corilagin which has anticancer activity .
Therefore, this plant recommended can be used as an alternative medicine in treating a disease such as DHF.
Based on the data obtained, the average examination results of platelet, erythrocyte and hematocrit counts increased with increasing doses.
Hematocrit itself is related to blood viscosity.
The higher the hematocrit value, the higher the blood viscosity.
High or low hematocrit values can cause an increase or can slow blood flow in capillaries.
According to these results.
niruri at the test dose will not trigger plasma permeation in dengue fever patients.
thrombocytopenia, the value of erythrocytes decreases, so according to the results obtained.
niruri also has activity in increasing the number of blood erythrocytes.
niruri is also proven to have an activity in increasing the platelet counts.
The increase in platelet count can occur due to the presence of bioactive flavonoid and tannin compounds as anti-aggregation of platelets to inhibit the formation of blood clots.
As well as from the results of the phytochemical screening, alkaloids, polyphenols, tannins, saponins, flavonoids, triterpenoids, and quinones were found in the extract.
In addition, flavonoids can also inhibit the enzyme hyaluronidase which functions in the degradation of hyaluronate.
Hyaluronic acid in the bone marrow can secrete mast cell interleukin (IL)-6 to induce the liver to secrete thrombopoietin and stimulate megakaryocytes so that platelet cells increase.
A study done previously also supports this finding.
Instead of ethanol extract, this study used aqueous extract of P.
niruri that has proven to be active in increasing the platelet counts in white rats that have phenytoin-induced thrombocytopenia.
paniculata contains several compounds, such as phenols, alkaloids, saponins, flavonoids, farnesol, and .
paniculata is often used as herbal medicine for various condition, from sepsis to anemia.
Based on previous research.
paniculata also has activity, especially in inhibiting Dengue Virus type 1 (DENV.
Dengue virus infection can cause platelet deficiency or known as thrombocytopenia.
Based on the data obtained.
paniculata has an effect in increasing platelet count with an average percentage increase at a dose of 500 mg/kg BW is 47.
paniculata is the third highest order of plants that can increase the platelet count of white rats.
paniculata has several bioactive compounds, .
From the results of the phytochemical screening, alkaloids, polyphenols, saponins, flavonoids, monoterpenoids, sesquiterpenoids, and quinones were found in the extract.
Andrographolide , other than a well known , is an effective anti-inflammatory agent .
, is also terpenoids that is an inhibitor of platelet activation by reducing the formation of hydroxy radicals (OH) initiated by collagen involving the activation of endothelial nitric oxide synthase-nitric oxide .
NOS-NO)/cyclic guanosine 3',5'-cyclic monophosphate (GMP) pathway and inhibiting PI3 kinase/Akt-p38 mitogen-activated protein kinase (MAPK) and phospholipase C gamma 2-protein kinase C (PLC2-PKC) cascade .
Therefore, andrographolide is the expected compound in A.
paniculata that are responsible for the anti-thrombocytopenic activity.
A previous study proves this hypothesis whereas both A.
extract and andrographolide are shown to have activity in increasing platelet counts.
However, the crude water extract of A.
paniculata shows slightly better platelet increasing activity than pure andrographolide.
Because of that, we assumed that while still be the most active andrographolide is not the only compound in A.
paniculata that has antithrombocytopenic activity.
From the experimental results of current study, almost all extracts gave an increase in platelets when the dose However, in C.
aeruginosa rhizome extract, the platelet counts at a dose of 250 mg/kg BW is greater than at a dose of 500 mg/kg BW.
This can happen because C.
aeruginosa rhizome extract does not depend on the dose If the dose is increased, the pharmacological activity is not optimal.
This research proves the empirical study of ethnomedicine in Indonesia, whereas plants that are often used by the community for DHF treatment do have antithrombocytopenic activity.
All plants, especially P.
ninuri, and A.
paniculata are potential to be further studied as novel anti-thrombocytopenic medications.
However, the limitation of this study is that the effective dose and active compounds of anti-thrombocytopenia from these three plants are unknown yet.
Therefore, further study will focus on obtaining effective doses and isolating active antithrombocytopenic compounds from P.
and A.
Nonetheless.
and A.
paniculata can be developed into pharmaceutical preparations and they may be further investigated as new anti-thrombocytopenic drugs.
DOI: 10.
18585/inabj.
Anti-thrombocytopenic Activity of Ethnomedicinal Plants from Indonesia (Susilawati Y, et al.
Indones Biomed J.
: 279-86
Conclusion From the results of testing the anti-thrombocytopenic activity of 15 plants, it is known that the three most potential extracts in increasing blood platelets are P.
guajava leaves .
82%).
22%), and A.
14%) at a dose of 500 mg/kg BW.
The three plants are recommended for handling dengue fever, as well as ethnomedicine studies, so it can be developed into herbal medicines with antithrombocytopenic activity Acknowledgments Authors thanks to research team: Dena Wijaya.
Whisda Mustika.
Dian Zahryanti.
Lani Hashina and Rayhan Zarra Safira, for their contribution on preparation the extract and pharmacological activity.
This research sponsored by DIPA with scheme KILAB 2021 Universitas Padjadjaran.
Authors Contribution YS.
EH and AT were involved in concepting and planning the research.
FFS and AT performed the data acquisition/ collection.
YS and EH calculated the experimental data and performed the analysis.
AA and HPD drafted the manuscript and designed the figures.
YS and EH aided in interpreting the results.
All authors took part in giving critical revision of the manuscript.
References