Jurnal Akademika Kimia, 10(1): 1-8, February 2021 ISSN (online) 2477-5185 | ISSN (print) 2302-6030 http://jurnal.fkip.untad.ac.id/index.php/jak/ OPEN ACCESS 1 Antioxidant Activity Test of Acetone and Ethanol Extracts of Cocoa (Theobroma cacao L.) Beans Husk *Siti S. K. Pandjo, Kasmudin Mustapa & Purnama Ningsih Pendidikan Kimia/FKIP – Universitas Tadulako, Palu – Indonesia 94119 Received 3 December 2020, Revised 7 January 2021, Accepted 1 February 2021 doi: 10.22487/j24775185.2021.v10.i1.pp1-8 Abstract Cocoa beans husk is waste produced from the cocoa processing industry containing alkaloid, flavonoid, tannin, saponin, and triterpenoid compounds. This material has the potential to be used as a source of natural antioxidant compounds. This study aimed to determine the antioxidant activity of cacao beans husk extracts by comparing acetone and ethanol as solvents through the extraction process. This antioxidant activity was determined using the DPPH (1,1-diphenyl-2-picrylhydrazyl) test method measured by UV-Vis spectrophotometer after adding an extract of cocoa beans husk. The positive control was vitamin C, while the negative control was DPPH solution dissolved in ethanol. The results showed that the extract of cacao beans husk has more potent antioxidant activity with a lower IC50 value of 181.2 ppm, while the acetone extract has an IC50 value of 247.9 ppm Keywords: Antioxidant, cocoa beans husk, DPPH, ethanol, acetone & Yenrina, 2015). Antioxidants are divided into Introduction two groups, namely synthetic and natural. Synthetic Cocoa (Theobroma cacao L.) is a antioxidants are obtained from chemical synthesis, plantation crop that produces cocoa beans and is while natural antioxidants come from the extraction one of the financial sources of farmers and the state. of natural ingredients that have the potential to capture free radicals (Isfahlan et al., 2010). Free Indonesia's cocoa plantations are approximately 1.72 million ha, with an average cocoa bean radicals are an atom or molecule that has unpaired production of about 776.880 tons. Sulawesi Tengah electrons. These paired electrons cause highly reactive free radicals that will then capture or take ranks first as a cocoa-producing region in Indonesia electrons from other compounds such as proteins, with a contribution of 156.63 thousand tons (21.69%) (Pusat Data dan Sistem Informasi lipids, carbohydrates, and DNA to neutralize themselves. Free radicals can enter the body and Pertanian, 2016). attack healthy cells causing them to lose their Sulawesi Tengah high cocoa productivity function and structure. The adverse effects of free encourages the government to build a chocolate radicals on the body can be prevented by processing industry. This chocolate processing antioxidant compounds (Sadeli, 2016). industry processes fermented cocoa beans from Antioxidant compounds from the skin farmers into semi-finished chocolate products, cocoa beans can be obtained by extraction, one of which are then sold and distributed to small and which is by the maceration method. Maceration was medium industries (SMEs) in Sulawesi Tengah. extraction using active compounds based on the Every industrial processing must produce waste in the process, as does this chocolate industry. The level of pattern, each solvent's ability to attract or bind to secondary metabolites contained in the processing process produces waste in the form of sample. The polar solvents will attract or bind to cocoa bean skin that is still underutilized. Cocoa active compounds that are polar and non-polar bean skin is likely to be used as a source of solvents will attract non-polar active compounds, or antioxidants because it contains polyphenol compounds with a total phenolic of 5.78% also called like dissolves like (Khopkar, 2010). Phenolic compounds have different affinity (Lecumberri et al., 2007). According to Kayaputri for solvent polarity properties Therefore, extracting et al. (2014), cocoa bean skin contains alkaloids, compounds in natural material tissues should be flavonoids, tannins, saponins, and triterpenoids, so used solvents of varying levels of polarity. The that the skin cocoa beans have the opportunity to be degree of polarity will determine the extraction yield used as a source of antioxidants. Antioxidant compounds are needed by the and antioxidant activity contained in the extract body to protect against free radical attacks (Sayuti (Taroreh et al., 2015). In general, water, ethanol,  *Correspondence: Siti S. K. Pandjo e-mail: sitisuhertina@gmail.com © 2021 the Author(s) retain the copyright of this article. This article is published under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. 1 Siti S. K. Pandjo et al. Alkaloids Filtrate attached to the KLT plate was then diluted with the eluent BAW (Butanol-Acetic acidWater) 4:1:5 then sprayed using a Dragendorf stain remover. The positive results will show orangebrown color (Wagner et al., 1984). methanol, acetone and ethyl acetate are solvents used to extract natural materials. Research related to the antioxidant activity of cocoa bean skin so far conducted with acetone solvents conducted by Utami et al. (2017) while for other solvents are still lacking, so that exploration of solvent types is needed in order to obtain better solvents to extract antioxidants in the cocoa beans skin. Testing of antioxidant activity is most common using the DPPH test (1,1-diphenyl-2pycrilhydrazyl). The working principle of this test is the measurement of antioxidant activity based on a measured decrease in DPPH absorption at a wavelength of 517 nm as a result of the presence of an antioxidant compound (Pisoschi et al., 2009). This paper presents testing of antioxidant activity in cocoa bean skin waste by comparing two types of solvents, namely acetone and ethanol, in producing extracts that have more potent antioxidant activity through the extraction process of cocoa bean skin. Flavonoids Filtrate attached to the KLT plate was then diluted with the eluent BAW (Butanol-Acetic acidWater) 4:1:5 then sprayed using AlCl3 stain remover. Positive results will show yellow color in UV rays with wavelengths of 366 nm (Wagner et al., 1984) Tannins The filtrate was attached to the KLT plate and then diluted with the eluent BAW (ButanolAcetic acid-Water) 4:1:5 then sprayed using FeCl3 stain remover 10%. The positive results will show a green-blackish color (Wagner et al., 1984). After checking the filtrate maceration results are combined, evaporated solvents using rotary vacuum evaporator on ethanol solvents used temperature 45 oC while solvent acetone used temperature 30 oC, so obtained concentrated extract of cocoa bean skin from ethanol solvents and acetone solvents. Method The tools used in this study are blenders, analytical balance sheet, measuring flask, Erlenmeyer flask, beath glass, measuring glass, test tube, test tube rack, stir bar, spatula, funnel, drip pipette, filter paper, aluminum foil, shaker, Buchner vacuum funnel, silica gel KLT plate 60 F 254, chamber, capillary pipe, rotary vacuum evaporator, and UV-Vis spectrophotometer. The ingredients used are cocoa bean skin, n-hexane (Merck), ethanol 90%, 90% acetone, Dragendorff reagents, Mg metals, HCl 2N, concentrated HCl (Merck), concentrated H2SO4 (Merck), chloroform (Merck), anhydrous acetate (Merck), FeCl3, AlCl3, distilled water, vitamin C, and 2,2-diphenyl-1-picrilhydrazil (DPPH). Qualitative Test Extract Each of the concentrated extracts before the antioxidant activity test is carried out a qualitative test to see the content of chemical compounds contained in the extract. Alkaloid Test 0.5 grams of the extract is dissolved in 5 mL HCl 2 N, then filtered. Filtrate is taken 1 mL then added 2 drops dragendorff reagents. A positive result with the formation of red deposits (Tiwari et al., 2011). Cocoa Bean Ari Skin Extraction 150 grams of cocoa bean skin powder extracted using n-hexane solvents as much as 225 mL in maceration for two days. Stirring is carried out for 1 hour at the time of immersion. On the second day, separated between residue and filtrate. The residue that has been separated was left for four days (Yumas, 2017). 20 grams of cocoa bean skin powder that has been fat-free is then moderated for 3 x 24 hours, each with 90% ethanol solvent and 90% acetone as much as 200 mL. After that, it is separated between the residue and the filtrate. The filtrate obtained first tested qualitatively using KLT then accommodated while the residue is pre-moderated until the solvent no longer shows discoloration (Departemen Kesehatan RI, 2000). The last filtrate that no longer shows the difference in color before being combined with other filtrates is rechecked to analyzed that the filtrate no longer contains antioxidant compounds that are expected by KLT in the following way: Flavonoid Test 0.5 grams of extract was dissolved in 5 mL ethanol. Then added 0.1 grams of magnesium metal and ten drops of concentrated HCl, when formed orange-red to purple-red indicates flavonoids or orange-yellow indicates the presence of flavons, kalkon, and auron (Fajriah & Megawati, 2015). Tannin Test 0.5 grams of extract dissolved in 5 mL ethanol, then added three drops of FeCl3 1%. If a bluish-black or green color is formed, then the sample positively contains tannins (Minarno, 2015). Saponin Test 0.5 grams of the extract was put in a test tube and added 10 mL of hot water, cooled, and shaken firmly for 10 seconds. If the foam was formed as high as 1 to 10 cm for not less than 10 minutes and at the addition of 1 drop of HCl 2N, the froth did not disappear, then the positive sample contains saponins (Fajriah & Megawati, 2015). 2 Volume, 10, No. 1, 2021, 1-8 Jurnal Akademika Kimia Triterpenoid and Steroid Tests 0.5 gram of the extract was put into the test tube, then added 2 mL chloroform and ten drops of anhydrous acetate, and three drops of concentrated H2SO4. A positive reaction is indicated by the formation of a red or purple-red solution for the indication of triterpenoids then a green-blue solution color for steroid indications (Harborne, 1987). The parameter commonly used to interpret the results of THE DPPH test was the efficient concentration (EC50) or often called IC50, which is the concentration that causes the loss of 50% of DPPH activity (Molyneux, 2004). The IC50 value was calculated using the percent inhibition of the test was performed. Percent inhibition can be calculated using the formula: Antioxidant Activity Test with DPPH Test % Making Solution DPPH 50 ppm 5 mg of DPPH was dissolved with ethanol in a 100 mL measuring flask, then sufficient in volume with ethanol to the boundary mark line. The sample concentration and percent of inhibition obtained were plotted respectively on the x and y axes on linear regression equations. The equation is used to determine the IC50 value of each sample stated with a value of y of 50 and x value to be obtained as IC50. The IC50 value represents the concentration of sample solution needed to reduce DPPH free radicals by 50% (Nurjanah et al., 2011). IC50 value of <50 ppm indicates very active antioxidant power, IC50 value of 50-100 ppm indicates active antioxidant power, IC50 value of 101-250 ppm indicates moderate antioxidant strength, IC50 value of 250-500 ppm indicates weak antioxidant strength, and IC50 value >500 ppm indicates inactive antioxidant power (Jun et al., 2003). Making Blanko Solution A 50 ppm DPPH solution of 2 mL pickpocket was put into the test tube then added ethanol 2 mL and covered with aluminum foil then homogenized and silenced for 30 minutes. Making a Test Solution for Cocoa Bean Ari Skin Extract 12.5 mg of each cocoa bean skin extract put into two 25 mL measuring flask. The first measuring gourd is added with ethanol to the limit mark, and the second measuring flask is added with acetone to the limit mark so that a master solution with a concentration of 500 ppm is obtained. Furthermore, each master solution is made of series concentrations of 20, 40, 60, and 80 ppm, respectively. Results and Discussion Cocoa Bean Ari Skin Extraction The extraction of cocoa bean skin is done through two stages of extraction. The first stage is extracted using n-hexane solvents to produce skin powder fat-free cocoa beans. At this extraction, the phase obtained 95.61 g of fat-free powder cocoa bean skin and concentrated extracts of cocoa bean skin using solvent acetone and ethanol as much as 10.25 and 10.01 g, respectively. The extraction process is carried out in a cold way that is maceration. This method is based on immersion of the sample inside the solvent so that the solvent will penetrate the cell wall and into the cell cavity containing the active compound. The active compound will dissolve in the appropriate solvent due to the difference in concentration between the active substance in the cell and outside the cell so that the adjacent solution is jostling and pushed out. The event lasts continuously until there is a concentration equilibrium between the solution outside and the one in the cell (Voight, 1995). Maceration is chosen because of its easy workmanship, simple use of tools and can minimize the influence of temperature in the extraction process. Before the third filtrate maceration process is stopped, checking using KLT, to ensure that the filtrate no longer contains the desired antioxidant compounds as a sign that the solution equilibrium has been achieved so that the maceration repeating process can be stopped; in this check, all compounds look negative on the extract of acetone skin cocoa beans, while in ethanol extract of cocoa bean skin Making Vitamin C Comparing Solution 12.5 mg of vitamin C was added with a little aquades and then sufficient volume up to 25 mL so that the main solution of vitamin C with a concentration of 500 ppm is obtained. Furthermore, each solution was made of series concentrations of 20, 40, 60, and 80 ppm, respectively. Absorption Measurement Spectrophotometer using 100 UV-Vis Measurement of Blanko Solution Absorption The blank solution is inserted into the cuvette and then measured absorption at a wavelength of 517 nm. Test Solution Absorption Measurement Each 2 mL of test solution inserted into the test tube was added with 2 mL of DPPH solution, homogenized, then silenced for 30 minutes, and put into the next cuvette measured absorption at a wavelength of 517 nm. Measurement of Vitamin C Comparison Solution Absorption Each 2 mL of comparative solution is inserted into the test tube added with 2 mL of DPPH solution, homogenized, then silenced for 30 minutes, and put into the cuvet then measured its absorption at a wavelength of 517 nm. 3 Siti S. K. Pandjo et al. there are still positive results for alkaloid compounds. This means that the extraction with ethanol solvents has not reached equilibrium for alkaloid compounds so that the remuneration process can still be done again if you want to optimize the extracted compounds. between tannins and Fe3+ ions forming complex compounds that indicate such discoloration (Harborne, 1987). Both cocoa bean skin extracts, namely ethanol extract and acetone extract showed positive results that are blackish green for ethanol extract and blackish blue color for acetone extract. Qualitative Test Extract Qualitative tests were conducted on concentrated extracts of cocoa bean skin for alkaloid compounds, flavonoids, tannins, saponins, triterpenes, and steroids Saponin Identification This saponin test using the forth test with positive results is shown by the presence of stable foam not less than 10 minutes and after the addition of HCl 2N. The onset of the foam indicates the presence of glycosides that have the ability to form foam in hydrolyzed water into glucose and other compounds (Rusdi, 1990). Both cocoa bean skin extracts, namely ethanol extract and acetone extract, showed negative results. Identification of Alkaloids Alkaloid testing used dragendorff reagents. The positive test result using this reagent is the obtaining of dark brown to yellow deposits. In the manufacture of Dragendorff reagents, bismuth nitrate is dissolved with acid so that there is no hydrolysis reaction because bismuth salts are easily hydrolyzed to form bismutil ions (BiO+). The addition of this acid serves to keep Bi3+ ions in the solution so that the equilibrium shifts to the left. Bi3+ ions then react with potassium iodide forming black deposits of bismuth(III) iodide, which then in excess potassium iodide form potassium tetraiodobismutat (Svehla, 1990). Alkaloids contain nitrogen that has a pair of free electrons so that it can be used to form coordinate covalent bonds with metal ions. In alkaloid tests with Dragendorff recalculation, it is estimated that nitrogen is used to form covalent coordinate bonds with K+, which are metal ions forming sediment; this deposit is potassium-alkaloid (Marliana et al., 2005). The results showed that both extracts of cocoa bean skin positively contain alkaloids, but in ethanol extract, there are deposits while acetone extract only occurs a change in the color of the solution to red not until sediment is formed. Identification of Triterpene and Steroids Triterpene and steroid testing using Lieberman-Burchard test is a mixture of acetate anhydride and concentrated H2SO4 that gives a green-blue color. Positive results were shown by the formation of a red or purple-red solution for triterpenoid indications and then green-blue solution color for steroid indications (Harborne, 1987). The results showed that both cocoa bean skin extracts were negative for steroid compound content because there was no visible discoloration of the solution to green-blue; however, acetone extract showed positive results for triterpene compounds due to the formation of purple-red solution in the solution while in ethanol extract showed negative results. After a qualitative test on cocoa bean skin extract, it was seen that extracts from acetone solvents have positive results in 4 types of compounds: alkaloids, flavonoids, tannins, and triterpenes. While extracts from ethanol solvents only have positive results in 3 types of compounds, namely alkaloids, flavonoids, tannins. These compounds can be extracted according to the same level of pattern as the solvent. According to Saifudin (2014), most terpenoid compounds have non-polar properties so that they can dissolve in non-polar and semi-polar solvents; therefore, triterpene compounds can be extracted in acetone solvents that have semipolar properties. However, other secondary metabolite compounds mostly have semipolar and polar properties whose solubility depends on the number of carbon chains and hydroxyl groups of each type. Identification of Flavonoids This flavonoid test using the Wilstater test shows orange or red-yellow, which means the positive presence of flavonoids. Magnesium and HCl in the Wilstater test formed H2 gas bubbles, while Mg metal with concentrated HCl in this test served to reduce benzopyran core contained in flavonoid structure so that discoloration becomes red or orange (Marliana et al., 2005). A plant extract containing flavonoids will form flavilium salts when the addition of Mg and HCl are red or orange (Achmad, 1986). Both cocoa bean skin extracts, namely ethanol extract and acetone extract, showed positive results that are orange for ethanol extract and red color for acetone extract. Antioxidant Activity Test with DPPH Test The implementation of the antioxidant activity test of cocoa bean extract is carried out using DPPH test method. This method was chosen because it is simple, easy, fast, and sensitive and requires very few samples (Molyneux, 2004). This method of testing is based on the ability of these antioxidant compounds to dampen free radicals. Free radicals used are DPPH (1,1–diphenyl– 2picrylhydrazyl). Tanin Identification Tannin testing using iron(III) chloride reaction. The result of this positive reaction test is the formation of blackish-green or blackish blue. The addition of FeCl3 1% in a positive extract containing tannins will show a blackish-green or blackish blue color due to the reaction that occurs 4 Volume, 10, No. 1, 2021, 1-8 Jurnal Akademika Kimia DPPH is a stable free radical due to the localization of electrons throughout the molecule. The localization of free electrons in these molecules causes the onset of the thick purple color of DPPH. If the solution of DPPH is mixed with a substance that can contribute hydrogen atoms, then the resulting reduced form of DPPH is accompanied by reduced intensity of purple color solution (Pisoschi et al., 2009). Observations on color intensity in this study were conducted on cocoa bean skin extract in the form of solutions made in several concentration variations, namely 20, 40, 60, and 80 ppm with duplo repetition. The solution is then reacted with a solution of DPPH (1,1–diphenyl– 2picrylhydrazyl) and incubated for 30 minutes in dark conditions at room temperature. The incubated solution is then measured using a UV-Vis spectrophotometer at a wavelength of 517 nm (Molyneux, 2004). Absorbansi 1.5 1 Ekstrak etanol 0.5 Ekstrak aseton 0 0 20 40 60 Concentration (ppm) 80 100 Figure 1. Relationship between absorption and concentration The measurement of absorbance from cocoa bean extract and vitamin C can be seen in Figure 1. The results showed that the absorption value of DPPH is decreasing as the concentration of cocoa bean extract increases. The same is also indicated by vitamin C. This decrease in absorption occurs due to the reduction reaction in DPPH by antioxidant compounds; according to the principle of this method, DPPH is reduced by the process of hydrogen or electron donation so that the color changes from purple to bright yellow comparable to the amount of electron donation (Dris & Jain, 2004). The change in color intensity is related to the number of DPPH electrons that capture hydrogen atoms from antioxidant compounds (Molyneux, 2004). The reaction between DPPH and antioxidant compounds can be seen in Figure 2. Figure 2. Reactions between DPPH free radicals and antioxidant compounds (Windono, 2001) The reduced intensity of purple color, along with the increasing concentration value of cocoa bean skin extract, indicates an increased antioxidant ability to capture DPPH free radicals (Molyneux, 2004). The results of absorbance measurements are then used to determine the percentage of free radical inhibition by skin extracts cocoa beans and vitamin C at various concentrations. A comparison of the percentage value of free radical inhibition by cocoa bean extract and vitamin C can be seen in Figure 3. Based on Figure 3, we can see that the percent of vitamin C inhibition is greater than that of both cocoa bean skin extracts and then followed by cocoa bean skin extract with ethanol solvents that are greater than the skin extract cocoa beans with acetone solvents. The difference of percent inhibition between cocoa bean extract and vitamin C looks quite significant; this can happen to look at the qualitative test results of cocoa bean skin extract, which still shows negative results in some types of antioxidant compounds tested. This certainly affects the bland power of the cocoa bean extract itself. In addition, it is also caused by vitamin C is a pure compound, while the extract of cocoa bean skin is a mixed compound, where the possibility of the presence of compounds that are not antioxidants that can affect the blandness of the skin extract cocoa beans. 5 Siti S. K. Pandjo et al. 50 Persentase Inhibisi 40 30 Ekstrak etanol Ekstrak Aseton 20 10 0 20 40 60 80 Konsentrasi (ppm) Figure 3. Profile comparison percentage of inhibition extracts and vitamin C The parameter commonly used to interpret the results of DPPH test is the efficient concentration (EC50) or often called EC50, which is the concentration that causes the loss of 50% of DPPH activity. The smaller the EC50value indicates, the greater the antioxidant activity (Molyneux, 2004). The EC50value is obtained by graphing between the percent value of inhibition and the concentration value obtained from the DPPH free radical inhibitor test. The percent value of inhibition is entered on the X axis and the concentration value is entered on the Y axis in one whole relationship graph (Nurjanah et al., 2011). Graph of the relationship between percent inhibition and concentration of cocoa bean extract can be seen in Figures 4 and 5 while for vitamin C in Figure 6. Perscent inhibition 30 25 20 persen inhibisi ekstrak etanol 15 10 Linear (persen inhibisi ekstrak etanol) 5 0 0 20 40 60 80 100 Consentration (ppm) Figure 4. Relationship between percent inhibition and concentration of ethanol extract of cocoa bean skin Percent inhibition 20 15 persen inhibisi ekstrak aseton 10 Linear (persen inhibisi ekstrak aseton) 5 0 0 20 40 60 80 100 Concentration (ppm) Figure 5. Relationship between percent inhibition and concentration of acetone extract of cocoa bean skin Percent Inhibition 20 15 persen inhibisi ekstrak aseton 10 Linear (persen inhibisi ekstrak aseton) 5 0 0 20 40 60 80 100 Concentration (ppm) Figure 6. Relationship between percent inhibition and vitamin C concentration 6 Volume, 10, No. 1, 2021, 1-8 Jurnal Akademika Kimia antioxidant activities of isoflavonoids from kudzu root (Puereria labata ohwl). Journal of Food Science, 68(6), 2117–2122. Kayaputri, I. L., Sumanti, D. M., Djali, M., Indiarto, R., & Dewi, D. L. (2014). Kajian fitokimia ekstrak kulit biji kakao (Theobroma cacao L.). Chimica at Natura Acta, 2(1), 83–90. Khopkar, S. M. (2010). Konsep dasar kimia analitik. Jakarta: UI Press. Lecumberri, E., Mateos, R., Pulido, Maria, I., Ruperez, P., Goya, L., & Bravo, L. (2007). Dietary fibre composition, antioxidant capacity and physico-chemical properties of a fibre-rich product from cocoa (Theobroma cacao L.). Food Chemistry, 104(3), 948–954. Marliana, S. D., Suryanti, V., & Suyono. (2005). Skrining fitokimia dan analisis kromatografi lapis tipis komponen buah labu siam (sechium edule jacq. swartz.) dalam ekstrak etanol. Biofarmasi, 3(1), 26–31. Minarno, E. B. (2015). 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Tetumbuhan sebagai sumber bahan obat. Padang: Pusat Penelitian Universitas Andalas. Sadeli, R. A. (2016). Uji aktivitas antioksidan dengan metode DPPH ekstrak bromelain buah nanas (Ananas comosus (L.) Merr.). Skripsi Tidak Diterbitkan. Yogyakarta: Universitas Sanata Dharma. Saifudin, A. (2014). Senyawa alam metabolit sekunder. Yogyakarta: Deepublish. Sayuti, K., & Yenrina, R. (2015). Antioksidan alami dan sintetik. Padang: Andalas University Press. IC50 value obtained from ethanol extract calculations, acetone extract of cocoa beans, and vitamin C, respectively, are 181.2 ppm, 247.939 ppm, and 100.191 ppm. IC50 < 50 ppm values show highly active antioxidant power, IC50 values of 50100 ppm show active antioxidant strength, IC50 values of 101-250 ppm show moderate antioxidant strength, IC50 values of 250-500 ppm show weak antioxidant strength, and IC50 > 500 ppm values show inactive antioxidant power (Jun et al., 2003). Vitamin C, as a comparison, has IC50 values ranging from 50-100 ppm, which are categorized as powerful antioxidants. Meanwhile, cocoa bean skin can be categorized as a natural antioxidant that has moderate activity because the IC50 value ranges from 101 - 250 ppm. However, when both cocoa bean skin extracts are compared then, cocoa bean skin extract with ethanol solvents has more potent antioxidant activity than cocoa bean skin extract with acetone solvent because the smaller the IC50 value indicates, the more potent its antioxidant activity (Molyneux, 2004) Conclusions Cocoa bean skin extract using ethanol solvents has more potent antioxidant activity with IC50 value of 181.2 ppm while 247.939 ppm for acetone extract of cocoa bean skin. Both fall into the category of moderate antioxidant activity. 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