Qanun Medika Vol.
3 No.
Januari 2019
Laporan Hasil Penelitian THE EFFECT OF PAGODA LEAF EXTRACT (CLERODENDRUM PANICULATUM L) ON THE IL-10 LEVEL IN MAMMAE OF FEMALE
Laporan Hasil Penelitian RATS STRAIN
(SPRAGUE DAWLEY) INDUCED WITH STAPHYLOCOCCUS
AUREUS BACTERIA
Israini Israini Suriati Suriati1.
Mardiana Mardiana Ahmad Ahmad2.
Nurul Nurul Aini Aini Siagian Siagian3.
Prihantono Prihantono4 Midwifery Midwifery Academy Academy Muhammadiyah Muhammadiyah Palopo Palopo Midwifery Academy Muhammadiyah Palopo Midwifery Academy Muhammadiyah Palopo Fakultas Fakultas Kebidanan Kebidanan Deli Deli Husada Husada Delitua Delitua Medan Medan Clinical Clinical Pathology Pathology Department Department Medical Medical Faculty Faculty Hasanuddin Hasanuddin University University Makassar Makassar Submitted Agustus : Desember Accepted : Januari Published : Januari Submitted:
Desember Januari Submitted:
Agustus 20182018Accepted:
Accepted:
Desember 20182019Published:
Published:
Januari Qanun Medika vol.
ulan tahun ABSTRAK
ABSTRAK
ABSTRACT
Mastitis Mastitis adalah adalah masalah masalah penyakit penyakit infeksi infeksi yang yang ditemukan ditemukan pada pada ibu ibu menyusui menyusui yang yang biasanya Mastitis disebabkan oleh oleh Staphylococcus Staphylococcus aureus Ketika Ketika bakteri bakteri Staphylococcus Staphylococcus aureus aureus masuk masuk ke Staphylococcus When Staphylococcus bodyAos IL-10 tubuh, kekebalan kekebalan IL-10 IL-10 akan akan aktif aktif membantu membantu mencegah mencegah selsel- sel sel rusak.
Sistem Sistem imun imun akan immune will prevent cell The immune will get worse if there is no Sehingga tidak helped ada penanganan.
Sehingga The daun pagoda, pagoda, kandungan kandungan dari dari tanaman tanaman daun daun pagoda pagoda terdiri terdiri dari dari tannins, tannins, alkaloids alkaloids and and flavonoids.
Jenis di mana senyawa ini dapat meningkatkan sistem kekebalan tubuh.
Jenis penelitianimmune This IL10 digunakan dalam dalam penelitian penelitian ini ini adalah adalah true true experimental experimental dengan dengan rancangan rancangan pre pre dan dan posttest Staphylococcus The control design.
Yang Yang menggunakan menggunakan tikus tikus strain strain Sprague Sprague Dawley Dawley sebanyak 15 ekor ekor yang yang dibagi with pre posttest control use pro Sprague Dawley 33 kelompok .
qua 55 ekor negatifThe .
qua 1ml/gramBB) semuanya diberikan diberikan pakan pakan standar, standar, kelompok kelompok kontrol kontrol positif positif (Amoxicilin (Amoxicilin dengan dengan dosis (Amoxicilin 9,6ml/250gramBB) 9,6ml/250gramBB) sebanyak 5 ekor semuanya diberikan pakan standar, dan kelompok (Amoxicilin perlakuan (Amoxicilin (Amoxicilin 9,6ml/250gramBB 9,6ml/250gramBB ditambah ditambah dengan dengan ekstrak ekstrak daun daun pagoda 50ml/250gramBB) All were induced .
ml/250gramBB) Semua .
ml/250gramBB) 55 ekor ekor semuanya semuanya diberikan Semua Staphylococcus mlx10Pemeriksaan ml/CFU).
IL-10
examined using ther& Staphylococcus ml/CFU.
IL-10
Staphylococcus 0,2.
ml/CFU.
Pemeriksaan IL-10
Enzyme
Linked Immonosorbent Assay
(ELISA)
Rat Data D system system Enzyme Enzyme Linked Linked Immonosorbent Immonosorbent Assay Assay (ELISA) (ELISA) Rat.
Rat.
Analisis Analisis data data menggunakan menggunakan uji ANOVA post post hoc, hoc test, a significant 0, 05.
This study showed no difference ANOVA
AA OO A0,
Penelitian ANOVA 0,O05.
Penelitian ini menunjukkan tidak ada levels of IL10 Staphylococcus bacteria in allaureus Staphylococcus sitokin IL-10.
IL-10.
Staphylococcus
(A>
Whereas <0.
Sedangkan Staphylococcus kelompok dengan nilai (A > 0,.
Sedangkan setelah diinduksikan bakteri Staphylococcus
that there in IL10mengalami levels after In the AA 24
IL10
jama semua IL10alldengan (A >>negative 0,.
IL10
ean While in IL-10 Setelah Setelah diberikan diberikan treatment treatment diperoleh diperoleh nilai nilai AA << 0,.
antara antara semua semua kelompok kelompok hal hal ini ini menunjukkan was greater the treatment group .
ean A 58.
pg / m.
, to the Pada kadarinIL-10
IL-10
setelah diberikan Pada .
ean The (Clerodendrum kontrol negatif negatif kadari kadari IL-10 IL-10 mengalami mengalami penurunan penurunan .
erata AA 10,4 10,4 pg/m.
pg/m.
Sedangkan Sedangkan peningkatan Paniculatum
IL-10
anti-inflammatory.
Pagoda
IL-10
erata pg/m.
, kadar IL-10 lebih besar pada kelompok perlakuan .
erata A 58.
4 pg/m.
, dibandingkan kelompok positif positif .
erata AA 41.
8 pg/ml pg/ml ).
Kesimpulan Kesimpulan dalam dalam penelitian penelitian ini ini adalah adalah ekstrak
ekstrak daun
pagoda (Clerodendrum (Clerodendrum Paniculatum
Paniculatum L) L) memiliki memiliki pengaruh pengaruh dalam dalam meningkatkan meningkatkan kadar kadar IL-10
IL-10
Keywords : Staphylococcus aureus ,dijadikan IL-10.
Ekstrak Daun Pagoda sebagai anti-inflamasi.
anti-inflamasi.
Ekstrak Ekstrak daun daun pagoda pagoda dapat dapat dijadikan terapi terapi komplementer.
Correspondence : israinisuriati@yahoo.
Kata Kata kunci Korespondensi Korespondensi :: Staphylococcus Staphylococcus aureus, aureus.
IL-10.
IL-10.
Ekstrak Ekstrak Daun Daun Pagoda Pagoda :: israinisuriati@yahoo.
israinisuriati@yahoo.
Midwifery Academy Muhammadiyah Palopo Midwifery Academy Muhammadiyah Palopo Fakultas Kebidanan Deli Husada Delitua Medan Clinical Pathology Department Medical Faculty Hasanuddin University Makassar Submitted: Agustus 2018 Qanun Medika Vol.
3 No.
1 Januari 2019
Accepted: Desember 2018 Published: Januari 2019
ABSTRAK
Mastitis adalah masalah penyakit infeksi yang ditemukan pada ibu menyusui yang biasanya disebabkan oleh Staphylococcus aureus.
Ketika bakteri Staphylococcus aureus masuk ke jaringan tubuh, kekebalan IL-10 akan aktif membantu mencegah sel- sel rusak.
Sistem imun akan memburuk jika tidak ada penanganan.
Sehingga dibutuhkan terapi komplementer seperti tanaman daun pagoda, kandungan dari tanaman daun pagoda terdiri dari tannins, alkaloids and flavonoids, di mana senyawa ini dapat meningkatkan sistem kekebalan tubuh.
Jenis penelitian yang digunakan dalam penelitian ini adalah true experimental dengan rancangan pre dan posttest control design.
Yang menggunakan tikus strain Sprague Dawley sebanyak 15 ekor yang dibagi dalam 3 kelompok yaitu kelompok kontrol negatif qua pro injection.
sebanyak 5 ekor
INTRODUCTION
semuanya diberikan pakan standar, kelompok Breast kontrol inflammation positif (Amoxicilin or dengan 9,6ml/250gramBB) sebanyak 5 ekor semuanya referred diberikanto aspakan dan kelompok is a disease that can perlakuan (Amoxicilin 9,6ml/250gramBB ditambah dengan ekstrak daun pagoda be accompanied by infection or not .
ml/250gramBB) 5 ekor semuanya diberikan pakan standar.
Semua kelompok diinduksisikan by infection, the type bakteri Staphylococcus aureus 0,2 ml/CFU.
Pemeriksaan kadar IL-10 of bacteria most often causes mastitisuji D system Enzyme Linked Immonosorbent Assay (ELISA) Rat.
Analisis data menggunakan ANOVA post hoc, dengan nilai signifikan A O 0,is05.
ini menunjukkan tidak ada thePenelitian Staphylococcus aureus bacteria.
perbedaan kadar sitokin IL-10, sebelum diinduksikanThis Staphylococcus pada semua kelompok dengan nilai (A > 0,.
Sedangkan setelah Staphylococcus lactation, so it is also called lactational aureus A 24 jam semua kelompok mengalami penurunan kadar IL10 dengan nilai (A > 0,.
mastitis or commonly referred to as Setelah diberikan treatment diperoleh nilai A < 0,.
antara semua kelompok hal ini menunjukkan This situation can be ada perbedaan kadar IL-10 setelah diberikan treatment antara semua Pada kelompok fatal ifA it10,4 is not Mastitis kontrol negatif kadari IL-10 mengalami penurunan .
erata pg/m.
Sedangkan kadar IL-10 lebih besar pada kelompok perlakuan .
erata A 58.
4 pg/m.
, dibandingkanpost will occur kelompok positif .
erata A 41.
8 pg/ml ).
Kesimpulan dalamThe ini adalah pagoda (Clerodendrum Paniculatum L) memiliki pengaruh kadar The IL-10 in mastitisdalam are abscess in the breast.
sebagai anti-inflamasi.
Ekstrak daun pagoda dapat dijadikan incidence of mastitis according to the world health Qanun Medika .
ulan tahun Kata kunci : Staphylococcus aureus.
IL-10.
Ekstrak Daunvol.
Pagoda more than 4.
1 million people are Korespondensi : israinisuriati@yahoo.
and an estimated (IL-.
iswith an mastitis, anti-inflammatory INTRODUCTION 3-20% of breastfeeding mothers can containing anti-inflammatory properties Breast inflammation or commonly mastitis, the 1 until 10 women that can suppress pro-inflammatory referred to as mastitis is a disease that can .
%) who experienced first in Interleukin mastitis 10 has inatherole be accompanied by infection or not after thedelivery.
The response signs and to accompanied by infection, while the type Fever in the to pathogens, thus preventing damage of bacteria that most often causes mastitis body, especially in the breast and tissues and maintaining connective tissue is the Staphylococcus aureus bacteria.
of levels the breast Interleukin-10 as antiThis disease usually accompanies accompanied by pain.
The causes of lactation, so it is also called lactational mastitis include: starting from being there is inflammation in the body (Couper mastitis or commonly referred to as by etbacteria.
N Kevin.
Rayitno nipples.
A, et al, puerperal mastitis.
This situation can be Garcia
YF,
Iyer fatal if it is not handled properly.
Mastitis Subramanian S, et al, 2012.
Kumar Ashok, generally occurs around 1 to 6 weeks post et al,breastfeeding AmirH.
Lisa,and et al, 2.
fast The complications that will occur weaning and attachment of babies to poor Inflammation when left without treatment in mastitis are abscess in the breast.
The breasts, and babies who only sucking incidence of mastitis according to the nipples .
ot including the areol.
causing world health organization estimates that the nipple to squeeze between the gums or neurodegenerative diseases, or cancer.
more than 4.
1 million people are lips so that the milk flow is imperfect for treatment so far which is still often diagnosed with mastitis, and an estimated (WHO, 2000: Tri Anasari.
Sumarni.
used for inflammation is conventional 3-20% of breastfeeding mothers can Amir H Lisa, et al, 2014: IDAI 2013.
Even though there are now experience mastitis, 1 until 10 women Anasary Tri Anasari.
Sumarni.
many herbal plants that can be used as .
%) who experienced mastitis in the first Dixon Michael J, et.
al 2011: Khanal complementary therapies, one of them is month after delivery.
The signs and Vishnu, et al.
2015: Viduedo Silva, et al Qanun Me (IL-.
i that can tissues an there is in N Kevin Subraman et al, 200 Inflamma for treatm used for many her the pago Shrivasta From the that pa (Joseph J pagoda le (M.
Prav From the the pago active co mmonly hat can or not he type to as can be Mastitis ks post ll occur The to the es that le are rs can the first ns and er in the st and uses of ncy or rly fast to poor gums or I 2013.
Khanal that pagoda plants contain active starting from being or (Joseph Jeenu.
From thetannins, results of such as:
infected by bacteria, scuffed nipples.
India alkaloids, phenolic acids, sterols,that weaning and attachment of babies to poor pagoda leaves also used as traditional and glycosides breasts, and babies who only sucking Korea,From for the HIV.
Syphilis, (Joseph Jeenu,in2.
ot including times,the Cancer.
Jaundice, the nipple squeeze between and to of babiesthe to gums Qanun Medika Vol.
3 No.
in1 India Januari (M.
Praveen.
Thakur, et al.
lips soand the milk onlyis sucking 2.
in Korea, for HIV.
Syphilis, (WHO.
Tri Anasari.
Sumarni.
the areol.
From the facts facts above which Cancer.
Jaundice, et between al, 2014:theIDAI theAmir nippleHto Lisa, on thet part (M.
Praveen, , 2012.
Thakur, , of Sumarni.
lipsAnasary so thatTri theAnasari, milk flow is imperfect the pagoda roots and flowers contain DixonTri Michael J, et.
al 2011:2014:
Khanal
(WHO,
Anasari.
Sumarni.
as antiFrom the various above which Vishnu, et al.
Viduedo et al Amir H Lisa, al, 2014:
IDAISilva, areof so that pagodagents plants on the part 2015: Tri Baratawidjaja K, et2014 al, .
Anasary Anasari.
Sumarni.
such as the pagoda roots and flowers contain Manzanillo Paolo.
J,etet.
al,al2.
Dixon Michael 2011: Khanal like compounds cancer, infections.
In anticertain that function Interleukin 10 (IL-.
Vishnu, et al.
Viduedo Silva, et al II Qanun Medika .
ulan areas the pagoda is strongly to be are so This typeK,of et has an Baratawidjaja al, 2014.
effective in curing various diseases such as binds to receptors Manzanillo Paolo,form et al,and that typhoid So In certain (IL-.
anti-inflammatory that are similar in structure.
Interleukin-10 Interleukin 10 (IL-.
type II areas the pagoda is strongly believed to be Thisanti-inflammatory type of cytokine properties has an a complementary therapy in certain that can suppress pro-inflammatory form and to receptors research were Sointhatconducting typhoid researchers Interleukin 10 Interleukin-10 has a role in are similar in structure.
limiting the immune response to of the penetration was the effect ofhalaman pathogens, thus preventing damage to extracts of pagoda leaves .
lerodendrum tissues and maintaining connective tissue paniculatum L) to the levels of il-10 in Interleukin-10 levels as antifemale mice induced by the bacterium inflammatory cytokines will decrease if staphylococcus aureus " besides that there is inflammation in the body (Couper pagoda leaves are available especially in N Kevin, et al 2008.
Rayitno A, et al.
South Sulawesi.
Elisa examination can be Garcia YF, et al, 2011.
Iyer done at the Hasanuddin University Subramanian S, et al, 2012.
Kumar Ashok.
Hospital in Makassar.
As well as research et al, 2000.
AmirH.
Lisa, et al, 2.
on the benefits of pagoda leaf extract as an Inflammation when left without treatment anti- inflammatory on mammae sprague Staphylococcus aureus bacteria has never neurodegenerative diseases, or cancer.
been done for treatment so far which is still often used for inflammation is conventional Even though there are now many herbal plants that can be used as complementary therapies, one of them is the pagoda leaf plant (Clerodendrum paniculatum L: Hafiz Ihsanul, et al 2.
Shrivastava, et al, 2.
From the phytochemical test results found that pagoda plants contain active compounds such as: flavonoids, tannins, alkaloids, phenolic acids, sterols, glycosides, phenolic acids, and glycosides (Joseph Jeenu, 2.
From the results of research conducted in India showed that pagoda leaves are also used as traditional medicine in Korea, for HIV.
Syphilis, typhoid.
Cancer.
Jaundice, hypertension (M.
Praveen, et al.
, 2012.
Thakur, et al.
From the various facts facts above which suggest that pagoda plants on the part of the pagoda roots and flowers contain
METHODS
The type of research used in this study is pure experimental or true experimental, which is an experiment conducted in the laboratory, with pre and post test control The group was divided into 3 .
groups: negative control group, positive control group, treatment group.
This research was carried out in the UNHAS Hospital Enzyme Linked Immunosorbent Assay (ELISA) and culture of Staphylococcus aureus bacteria.
Biopharmaca laboratory for drying plants.
Pharmacy Laboratory of State Islamic University (UIN) Makassar to extract and phytochemical testing, 65 animal laboratory UNHAS for the process of rats adaptation to the end of the treatment given.
The sample in this study were Sprague Qanun Med 50ml/250g The instrum materials, na Luwu.
Sou people's hou the UNHA previously th kg of raw le then sifted Aft extracted by obtained A 5 rats strain S 250 grams.
or negative (For positive Staphylococ making a sus 10 ml mix bacteria col turbidity lev Accuracy o measured by dosage is inj 0,2 mlx108 m is carried ou the breast p duct in rats i and treatmen is then obse infection re (There are si The research carried out Immunosorb IL-10 levels.
earch for e the title of giving f il-10 in sides that ecially in on can be University s research tract as an e sprague has never s study is ted in the st control d into 3 ol group, ut in the Linked SA) and atory for ratory of Makassar he process d of the Sprague of about rats strain Enzyme Linked Accuracy of turbidity level Mc Farland positive control group, treatment group.
is carried out by intramuscular technique in Immunosorbent Assay (ELISA) and measured by the Densi check tool.
So that the This research was carried out in the the breast part precisely in the lactiferous culture of Staphylococcus aureus dosage is injected into as many rats as possible duct in rats in all8 groups (Negative, positive UNHAS Hospital bacteria.
Biopharmaca laboratory for 0,2 mlx10 ml/CFU.
Bacterial and treatmen.
the induction of bacteria Enzyme Linked drying plants.
Pharmacy Laboratory of carried out is thenis observed A24byhours later to see Immunosorbent Assay and Medika Qanun Vol.
3 No.
Januari State Islamic University (UIN)(ELISA) Makassar infection reaction in the rat breast area of phytochemical Staphylococcus to culture extract and in ratsofininfection all groups (Negative, (Thereduct are signs in the breas.
positive Biopharmaca UNHAS for laboratory the process for and treatmen.
after the of bacteria The research data collection plants.
Pharmacy Laboratory of drying rats adaptation to the end of the of A24 to see an carried out by method Enzyme Linked State Islamic University (UIN) Makassar infection Assay in theto rat breast area Immunosorbent (ELISA) to sample extractin and The this study were Sprague are signs of infection in atthe IL-10(There Histopathology test done UNHAS Dawley with a body The research data collection instrument was University Hasanuddin of the 200With usetoof the 15 rats Enzyme Makassar.
The tissue taken for the sample is Linked Sprague Dawley divided into 3 groups: 5 Assay (ELISA) to measure breastImmunosorbent tissue in all groups.
The sample in this study.
qua were Sprague IL-10 levels.
Histopathology test done This research was approved and issued by at the Dawley 1ml/gramBB) rats with a body of about all given University Hasanuddin the Ethical Commitment of the University 200- 250 Withno.
15 group Qanun Medika .
ulan Makassar.
The sample is Hasanuddin Makassar on February 5, 2018 Sprague Dawley (Amoxicilin ) as 50ml/250gramBB) 5/ 250gramBB rats into were3 all tissue/ inH4.
all groups.
/ PP36with breast No.
qua as 5 rats and pro all all KOMETIK / 2018.
was approved and issued by This research (Amoxicilin 1ml/gramBB) all include The used in this Data the is Ethical and analyzed Commitment of the using University of plus pagoda standard namely Belopa, to see on the February effect of 5, 2018 Qanun Me Hasanuddin Makassar (Amoxicilin 6ml / 250gramBB Luwu.
Southwith Sulawesi, precisely in ) the leaves/ H4.
(Clerodendrum/ PP36No.
many ashouse.
5 rats And all fedout L), to /levels KOMETIK of IL10 cytokines.
groupUNHAS feeding Biopharmaca (Amoxicilin laboratory.
IL-10 Data seen usingand the analyzed method using plus pagoda the pagoda leaves were A5 Enzyme Linkedassistance.
Immunosorbent to see Assay the effect of kg of raw leaves, after that it is cleaned first, (ELISA).
Three levels of IL-10 were then sifted to obtain a smooth simplicia examined .
time, blood collection firsthalaman After that the sample is ready to be when rats have not been treated to assess extracted by maceration method, so that it is whether IL-10 levels are normal or not, then obtained A 500 gram pagoda thick extract.
The taking blood the second time after the mouse experimental animals used in this study were was induced by Staphylococcus aureus rats strain Sprague Dawley with weight 200bacteria, and taking blood last time when 250 grams.
And needed Aqua pro injection mice were given good in the negative, .
or negative grou.
, and amoxicillin 500 mg, positive and treatment groups.
(For positive group.
, and the bacteria used are The bivariate test uses a test one way anova to Staphylococcus aureus bacteria strain see differences in IL-10 levels in the negative To make a sample of bacteria by control group before, during and after making a suspension in solution NaCl as much treatment, positive control before, during and 10 ml mixed with Staphylococcus aureus after being given treatment, and treatment bacteria colonies that golden yellow with group before, during and after being given turbidity level Mc Farland 2 x 108 CFU.
treatment and done.
But if the data is not Accuracy of turbidity level Mc Farland normally distributed then a test is carried out measured by the Densi check tool.
So that the Kruskal-Wallis.
other than that also done test dosage is injected into as many rats as possible repeted anova if the data is normally 0,2 mlx108 ml/CFU.
Bacterial induction distributed to see differences in IL-10 levels in is carried out by intramuscular technique in each group, namely the negative, positive, and the breast part precisely in the lactiferous treatment groups before, during and after duct in rats in all groups (Negative, positive being given treatment.
and treatmen.
after the induction of bacteria is then observed A24 hours later to see an infection reaction in the rat breast area (There are signs of infection in the breas.
The research data collection instrument was carried out by method Enzyme Linked Immunosorbent Assay (ELISA) to measure 66 IL-10 levels.
Histopathology test done at the University Hasanuddin Makassar.
The tissue taken for the sample is Qanun Medika .
ulan Qanun Medika .
ulan Qanun Medika Vol.
3 No.
Januari 2019
RESULTS
RESULTS
Table Phytochemical of the of Leaf Pagoda (Clorodendum Table Phytochemical of the of Leaf Pagoda (Clorodendum Paniculatum L).
Paniculatum L).
Sample Nama Sample Nama DaunPagoda DaunPagoda (Clorodendum (Clorodendum Paniculatum L).
L).
Paniculatum
Identification of Compound
Groups Identification of Compound
Groups Steroid/ Steroid/ Flavonoid
Tanin
Flavonoidtripernoid Alkaloid
Alkaloid
Tanin
-- --
Qanun Medika .
ulan tahun Describsion : .
are are of bioactive Describsion : .
of bioactive Table 2.
The mean body weight of mice in each group
Weight
Mean A SD
Min-Maks Nilai A 226 A 12
213 Ae 239
Negative control group
220 A 8
208 Ae 233
Poaitive control group
0, 56
220 A 10
208 Ae 231 Treatment Group *One way ANOVA.
Significant value A 5 0.
There were no differences in body weight in all groups, both in the negative, positive and treatment control groups (A = 0.
Table 3:Average differences in IL-10 levels in each female rat group Sprague Dawley strain before, after induction of Staphylococcus aureus bacteria and after treatment.
IL-10 levels .
g / m.
Before Induction Mean A SD After Induction Mean A SD After Treatment Mean A SD Value 111,2 A 10,5 34,5 A 8,2 24,1 A 5,6 115,1 A 16,9 33,7 A 8,2 75,4 A 7,6 Treatment (Antibiotic Amoxicilin 9,6ml/250grBB pagoda leaf extract50 ml/grBB .
111,9 19,6 31,8 16,1 90,3 11,4 0,003 Nilai A 0, 58b Group Negative Control .
Positif Control (Antibiotic Amoxicilin 9,6 ml/250grBB ) .
The results of the statistical test of one way ANOVA before induced Staphylococcus aureus bacteria in each group obtained the value A = 0.
58 greater than the value of = 0.
This means that there is no significant difference in the levels of IL-10 cytokines before induced Staphylococcus aureus bacteria between the negative, positive and treatment control groups.
Whereas IL-10 levels after induced Staphylococcus aureus bacteria A 24 hours in each group obtained values A = 0.
68 greater than the value of = 0.
Picture 1: Trend of IL-10 levels in each group of female rats with Sprague Dawly strain before, after induced Staphylococcus.
Aureus and after treatment.
leaf extract50 ml/grBB .
Nilai A 0, 58b The results of the statistical test of one way ANOVA before induced Staphylococcus aureus bacteria in each group obtained the value A = 0.
58 greater than the value of = 0.
This means that there is no significant difference in the levels of IL-10 cytokines before induced Staphylococcus aureus bacteria between the negative, positive and treatment control groups.
Whereas IL-10 levels Medika after inducedVol.
Staphylococcus bacteria A 242019 hours in Qanun 3 No.
1 Januari each group obtained values A = 0.
68 greater than the value of = 0.
Picture 1: Trend of IL-10 levels in each group of female rats with Sprague Dawly strain before, after induced Staphylococcus.
Aureus and after treatment.
Qanun Medika vol.
ulan tahun Description: IL-10 levels in each group before induced Staphylococcus aureus bacteria .
, and after induced Staphylococcus aureus bacteria all groups experienced a decrease in IL-10 levels.
Whereas after giving the treatment the positive control group and the treatment increased, the group while the negative control group decreased.
Table 4: Analysis of differences in IL-10 levels before, after induced Staphylococcus aureus
bacteria and after treatment in each group
Measurement Before Treatment
Negative Control
Positive Control
Negative Control
Treatment
Positive Control
Treatment
Mean A SD
111,2 A 10,5
115,1 A 16,9
111,2 A 10,5
120,4 A 16,7
115,1 A 16,9
120,4 A 16,7
After Inductions bakteria s.
Negative Control
34,5 A 8,2
Positive Control
Negative Control
33,7 A 8,2
34,5 A 8,2
Treatment
Positive Control
30,8 A 6,6
33,7 A 8,2
Treatment
After Treatment
Negative Control
30,8 A 6,6
Positive Control
Negative Control
75,4 A 7,6
24,1 A 5,6
Treatment
Positive Control
90,3 11,4
75,4 A 7,6
Treatment
90,3 11,4
24,1 A 5,6
Difference Mean
Nilai A* 1,00 0,92 1,00 1,00 1,00 1,00 Nilai 0,58 0, 68
0,00
Post hoc analysis before and after induced Staphylococcus 68aureus bacteria obtained a value of A> 0.
05 between each It showed no difference in levels of IL-10 cytokines, both before and after induction of A 24 hours of Staphylococcus aureus bacteria in all groups.
Whereas after being given treatment, the value of p <0.
05 was obtained between all groups.
This shows that there are differences in IL-10 levels after treatment between all groups.
QanunVol.
Medika 1 no.
Januari .
ulan tahun Qanun Medika 3 No.
Table 5:
Analysis Of Differences In Il-10 Levels Between Groups Of Female Sprague Dawley Strain Rats Before.
After Induced By Staphylococcus aureus Bacteria And After Treatment.
Value Value Mean A SD
Difference Measurement Mean
0,000
Negative Control Before After induction s.
111,2 A 10,5
34,5 A 8,2
Before After treatment
111,2 A 10,5
24,1 A 5,6
0,000
After induction s.
After treatment
Positive Control
34,5 A 8,2
24,1 A 5,6
0,049
0,000
0,008
-41,8
0,000
Before After induction s.
Before After treatment After induction s.
After treatment
Treatment
115,1 A 16,9
33,7 A 8,2
115,1 A 16,9
75,4 A 7,6
33,7 A 8,2
75,4 A 7,6
Before After induction s.
Before After treatment After induction s.
After treatment
111,9 19,6
31,8 16,1
111,9 19,6
90,3 11,4
31,8 16,1
90,3 11,4
0,000
0, 001
0,002
*Repetead ANOVA post hoc in the treatment group before and after being given treatment obtained the value A = 0.
64 greater than the value of = 0.
This shows no difference in levels of IL-10 cytokines before and after treatment in the treatment group.
Whereas in the negative and positive control groups obtained a value of A <0.
05, both before, after induction s.
aureus A 24 hours and after treatment.
Table 6: Analysis of Velocity in the positive control group and treatment group Group Persentase (%) Positive Control (Antibiotik Amoxicilin 9,6 mg/250grBB ) .
Treatment (Antibiotik Amoxicilin 9,6 ml/250grBB ekstrac Pagoda Leaft 50 ml/grBB .
Difference Persentase (%) Showing the results of velocity test in the positive control group obtained 133.
4% antibiotics can increase levels of IL10 cytokines, whereas in the treatment group obtained 271.
5% antibiotics and pagoda leaf extract can increase levels of IL-10 cytokines, with the difference between positive control groups and the treatment group was 138%.
This shows that pagoda leaf extract can increase the levels of IL-10 cytokines by around 138%.
Qanun Medika vol.
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Januari 2019 Picture 2 : Histopathology test results Picture 1.
Histopatologi normal (A) Tampak adanya pembuluh darah, (B) Tampak adanya sel epitel yang normal (C) Tampak adanya duktus laktiferus.
Picture 2.
Histopatologi Negative (A)Tampak adanya sel-sel radang PMN disekitar jaringan ikat (B)Tampak adanya sel radang disekitar lapisan sel epitel Picture 3.
Histopatologi Positive group Picture 4.
Histopatologi Treatmen (A)Tampak adanya sel-sel radang PMN yang (A)Tampak adanya sel-sel radang mengelilingi kelenjar susu PMN disekitar jaringan ikat (B) Tampak adanya sel radang disekitar jaringan ikat.
Qanun Medika Vol.
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Januari 2019 Qanun Medika vol.
ulan tahun Staphylococcus aureus bacteria the condition of mice in each group was not in a healthy condition.
After the rats were ascertained in a healthy condition, it was continued by inducing Staphylococcus aureus bacteria .
2 mlx108ml / CFU).
All groups were induced by bacteria so that the rats were exposed to bacteria.
In the negative control group, 1 ml / 200gram BB mice aqua pro injection was In this group, levels of IL-10 cytokines were decreased.
This is due to an immune response in the body called pro IL-10 cytokines are no longer able to suppress proinflammatory consequences, there is an inflammatory response or infection.
This is in line with research conducted by John M.
Leech who got interleukin-10 levels decreased after 24 hours after induced Staphylococcus aureus bacteria, so that mice will be Even up to 7 days the level of Interleukin 10 has decreased dramatically, if not given treatment such as antibiotics.
(Jawetz, 2005: Ziegler Christina, 2011: Li Huimin, et al, 2017: jawetz, 2005: Ziegler Christina, 2011.
John M.
Leech, 2017:
Manzanillo Paolo, et al, 2015: Ziegeler Christina, et al, 2.
(John Leech,2017: Manzanillo Paolo,et al, 2015: Ziegeler Christina,et al, 2.
In the positive control group, rats were induced by Staphylococcus aureus bacteria .
2 ml x 108 ml / CFU) and given Amoxicilin treatment with a dose of 6 mg / ml / 250 gBB mice for 5 days.
The results showed that there were differences in levels of IL-10 cytokines before bacterial induction and after antibiotic The result is clear that there are differences between groups before, during and after being given Amoxicilin treatment with a dose of 9.
/ ml / 250gramBB (S Jahanfar, et al.
WHO, 2000.
Guideline and Procedure Manual, 2012.
IDAI, 2013: Guidelines.
Mastitis And Breastfeeding, 2015: Katari Kamal, et al, 2012: The Women, 2.
Researchers thought that in the positive (Figure I) Microscopic picture of the breast of the normal control group showed that the lactiferous duct was coated with normal epithelial cells and surrounded by connective tissue and blood vessels (Figure II) Microscopic images of the mice of the negative control group, namely the group induced by Staphylococcus aureus bacteria without treatment, it was shown that there were inflammatory (A .
Polymorphonuclear (PMN) cells around the connective tissue which were not found in the normal control group.
It appears that thickening of the epithelial cell layer surrounding the lactiferous duct (Figure .
Microscopic picture of the breast of the mouse in the positive control group is the group induced by Staphylococcus aureus and given 9.
6ml / 250grBB of amoxilcillin antibiotics per day orally for 5 .
days showing the cell - inflammatory cells (A .
Polymorphonuclear (PMN) around the connective tissue and surrounding epithelial cells and reduced lactiferous ducts (Figure IV) Microscopic images in the mice breast of the treatment control group ie the group induced by Staphylococcus antibiotics amoxilcillin at a dose of 9, 6 ml / 250grBB pagoda leaf extract with a dose of 50 ml / grBB per day orally plus pagoda leaf extract 50 ml / g BB for 5 .
days and showed inflammatory cells (A .
Polymorphonuclear (PMN) around the connective tissue , the mammary gland and epithelial cells are reduced.
DISCUSSION